中文摘要：

水稻条纹病毒（rice stripe virus,RSV）NS3蛋白为病毒的沉默抑制子。利用酵母双杂交技术,从水稻cDNA文库中筛选出一个与RSV NS3蛋白互作的基因片段。推测该基因的功能是编码水稻3-磷酸甘油醛脱氢酶（glyceraldehyde-3-phosphate dehydrogenase,GAPDH）。双分子荧光互补实验进一步验证了NS3与GAPDH存在互作。瞬时表达实验表明,GAPDH与GFP基因融合蛋白在本氏烟表皮细胞细胞质中大量积累。此外,讨论了GAPDH蛋白在RSV侵染水稻过程中可能的功能。

英文摘要：

NS3 protein of rice stripe virus （RSV） is an RNA silencing suppressor. Yeast two-hybrid assay was used to screen the rice cDNA library with the NS3 protein as a bait. A novel rice protein interacting with RSV NS3 was obtained. The putative function of the interacting protein was glyceraldehyde-3-phosphate dehydrogenase （GAPDH）. Bimolecular fluorescence complementation （BiFC） also showed that RSV NS3 could interact with the GAPDH. Cellular localization studies showed that GAPDH-GFP fusion protein accumulated predominantly in cytoplasm of tobacco （Nicotiana benthamiana） through transient expression assay. Possible functions of GAPDH during the infection of rice by RSV were discussed.