中文摘要：

提出了一种优化设计方案。该方案的各项评价指标均优于根据以往文献提供的方法所能得到的最好结果。尤其是所提出的海明距离测度方法，进一步保证了特异性杂交产生的自由能远大于非特异性杂交所产生的自由能，便于进行DNA编码序列的设计与选择，为可控的DNA计算提供可靠有效的编码序列。

英文摘要：

Encoding sequences design is a crucial problem in DNA computing. Not only an optimized approach for constructing a set of encoding sequences was developed by using random generate and real-time filter algorithm, but several evaluation terms were proposed to compare with other existing sequence design systems. The comparison results show the performance of this approach outperforms the existing DNA sequence design systems, especially in preventing sequence serf-complementary from forming secondary structure and keeping the uniform melting temperature among sequences. It is convenient for user to design and select proper encoding sequences in silicon, and can provide reliable and effective encoding sequences for controllable DNA computing.