中文摘要：

目的研究依曲替酸对培养的正常人角质形成细胞（KC）增殖和肝素结合表皮生长因子样生长因子（HB-EGF）mRNA表达的影响。方法0.1和1μmol/L伊曲替酸处理正常人KC 12 h后，用噻唑蓝比色法（MTT法）和实时荧光定量逆转录聚合酶链反应（RT-PCR）法分别检测KC增殖和HB-EGF mRNA表达的改变。结果依曲替酸处理12 h后，可剂量依赖抑制KC的增殖和上调HB-EGF mRNA的表达。0.1和1μmol/L伊曲替酸分别使KC增殖抑制10.2%和14.4%，使HB-EGF mRNA增加到正常对照组的3.2和7.1倍。与对照组相比，差异均有统计学意义（P〈0.05）。结论依曲替酸可剂量依赖上调HB-EGF mRNA的表达，其可能参与依曲替酸对KC增殖的抑制作用。

英文摘要：

Objective To investigate the alteration of cell proliferation and heparin-binding epidermal-growth-factor-like growth factor（HB-EGF）mRNA expression in cultured normal human keratinocytes after acitretin treatment. Methods After a 12-hour incubation with 0. l and 1μmol/L acitretin in normal human keratinocytes, the proliferation potency of the cells was detected by MTT colorimetric assay and the expression of HB-EGF mRNA was examined by real-time quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results Both keratinocytes growth inhibition and HB-EGF mRNA expression was dose-dependently induced by acitretin. Treatment with 0. l and 1μmol/L acitretin inhibited keratinocyte proliferation by 10.2% and 14.4%, and elevated HB-EGF mRNA by 3.2-and 7.1-fold, respectively. Conclusion Upregulated HB-EGF mRNA expression induced by acitretin in normal human keratinocytes may be involved in regulating keratinocyte growth inhibition after acitretin treatment