中文摘要：

目的：探讨侧脑室注射orexins（食欲素）、NPY（神经肽Y）、MCH（黑色素聚集激素）和甘丙肽对大鼠摄食的影响及其机制。方法：将成年雄性Wistar大鼠随机分为对照组、侧脑室注射组和室旁核（PVN）注射组。通过套管将orexin-A、orexin-B、NPY、MCH和甘丙肽分别注射至侧脑室和PVN内,随后测量大鼠食物摄入量,并检测PVN、弓状核（ARC）和VMH内c-fos的表达。结果：与对照组比较,侧脑室注射NPY、MCH和orexin-B 2 h后,大鼠摄食量显著增多（P〈0.05）。相较于orexin-B和MCH,NPY对摄食的影响更显著（P〈0.05）。与NS对照组比较,侧脑室注射甘丙肽和orexin-A 1 h后,大鼠摄食量显著增多（P〈0.05）。与NS对照组比较,侧脑室注射orexin-A可显著增加c-fos在PVN和ARC中的表达,在VMH中效应较弱（P〈0.05）。与NS对照组比较,PVN注射NPY能显著增加大鼠2 h摄食量（P〈0.05）,PVN注射orexin-A能显著增加大鼠2 h和4 h摄食量（P〈0.05）。结论：orexins与可促进大鼠摄食,此效应可能通过下丘脑参与摄食调控中枢PVN和ARC而实现的。

英文摘要：

Objective： To investigate the role of the orexins, NPY, melanin-concentrating hormone（MCH） and galanin in food intake in rats and their mechanism. Methods： Male wistar rats were randomly divided into sham group, lateral ventricle（ICV） group and PVN group. Orexin-A, orexin-B, NPY, MCH and galanin were respectively injected into ICV and PVN through the cannula, then measured the food intake of rats to compared their effect. Also the expression of c-fos was detected in PVN, ARC and VMH. Results： Compared with sham group, the 2 h food intake of rats following injected NPY, MCH and orexin-B into ICV had significantly increased（P〈0.05）. The effect of NPY in food intake was more obviously than orexin-B and MCH（P〈0.05）. Compared with sham group, the 1 h food intake of rats following inject galanin and orexin-A into ICV had significantly increased（P〈0.05）. C-fos was expressed in PVN and ARC markedly（P〈0.05）. Compared with sham group, the expression of c-fos was greatly inhanced after orexin-A administered into ICV, weakly in VMH（P〈0.05）. Compared with sham group, the 2 h food intake of rats following injected NPY into PVN had significantly increased（P〈0.05）, orexin-A markedly increased the food intake of rats at 2 h and 4 h（P〈0.05）. Conclusions： Orexin-A can stimulated food intake of rats, and it may related to its physiology role in appetite regulation.