中文摘要：

目的 用双源CT冠状动脉CT血管造影（DSCT-CCTA）评价猪急性心肌梗死后诱导性多能干细胞（iPSc）移植对左心室心功能变化的影响。方法 将18只猪随机分入对照组、心肌梗死（AMI）组和iPSc移植组，每组6只。术后1周及6周分别应用DSCT-CCTA技术观察左心功能改变情况，检测其左心室舒张末期容积（LVEDV）、收缩术期容积（LVESV）和左室射血分数（LVEF），比较每组内前后变化及3组间差异；术后6周处死动物，取心脏标本行TTC染色和HE染色。结果 术后1周心肌梗死组及iPSc移植组较对照组LVEF降低（P〈0．0001），LVESV及LVEDV变化小大；术后6周心肌梗死组较对照组LVESV增加（P=0．010）．LVEF降低（P〈0．001），iPSc移植组较心肌梗死组LVEF升高（P=0．003）；术后6周和1周相比心肌梗死组LVESV、LVEDV均增加（t=3．86，P=0．012；t=2．96，P=0．031），LVEF未见明显变化，iPSc移植组左心室LVEDV及LVEF均增加（t=11．91，P=0．000；t=3．14，p=0．026），LVESV未见明显差异。结论急性心肌梗死iPSc移植6周后心功能明显改善，梗死面积有所减小。用DSCT-CCTA技术可以评价AMI后左心室重构过程及iPSc移植对左心功能的影响，对临床治疗及预后评价具有重要指导意义。

英文摘要：

Abstract：Objective To estimate the imaet of being implanted induced pluripotent stem cells （iPSc） on the left ventricular func- tion of acute myocardial infarction （AMI） swine model by using dual source CT-coronary artery CT angiography （DSCT-CCTA）. Methods Eighteen swine were randomly equally divided into control group,AMl group and iPSc group. The left heart function of 3 groups was assessed by DSCT at 1 and 6 weeks after operation, respectively, including the left ventricular end-systolic volume （LVESV）, left ventricular end-diastolic volume （LVEDV） and left ventricular ejection fraction （LVEF）. Surviving iPSc in infarcted myocardium were identified by triphenyltetrazolium chloride （TTC） stain and hematoxylin and eosin（HE） stain 6 weeks after the transplantation. Results Comparing with the control group, LVEF decreased （P〈0. 000 1 ） in the AMI group and iPSc group 1 week after operation. Six weeks later , the AMI group showed increased LVESV （P=0.010） and decreased I.VEF （P〈20.001） in relative to the control group, while , the iPSc group showed increased LVEF （P=0. 003） compared with AMI group. The AMI group showed increased I.VESV and I.VEDV （t=3.86,P=0.012;t=2.96,P=0.031） 6 weeks after operation compared with the first week＇s result but the LVEF showed no significant difference . The iPSc group showed increased I.VEDV and I.VEF （t=11.91, P= 0. 000;t= 3. 14,P 0. 026）, but the LVESV showed no significant difference 6 weeks after operation with the first week＇s resuit. Conclusion hnplantation of iPSc into the infracted myocardium might decrease the infarction area and improve cardiac function6 weeks after the implantation . DSCT-CCTA can be used to evaluateg left ventricular remodeling after acute myocardial infarction and the impact on the cardiac function of the implanta- tion of iPSc, and contribute to the clinical treatment and prognosis.