中文摘要：

本实验利用透析袋平衡透析、毛细管电泳Ru（bpy）3^2＋电化学发光检测技术测定了丙吡胺和人血浆蛋白的结合率。在恒电位1．3V；进样电压10kV持续10s，分离电压15kV，运行缓冲液30mmol／L磷酸盐缓冲液（pH7．5），检测池中为5mmoL／LRu（bpy）3^2＋稀释于50mmol／L磷酸盐缓冲液（pH7．5）中等最优化的条件下，丙吡胺的检出限为10μmoL／L（S／N=3）。对蛋白结合率的测定结果表明，人血浆中的药物浓度为1．6-8．2mmol／L，丙吡胺与血浆蛋白的结合是呈线性的，其线性回归方程为y=-0．07＋0．93x，线性相关系数r为0．9999，丙吡胺与人血浆蛋白的结合率约为90．4％。

英文摘要：

The binding rate of disopyramide with human plasma protein was determined using capillary electrophoresis（CE） coupled with tris（2,2＇-bipyridyl） ruthenium（ Ⅱ ） [ Ru（bpy）3^2＋ ] electrochemiluminescence （ECL） detection after equilibrium dialysis. Under optimal conditions（ ECL detection at 1.3 V, electrokinetically injection at 10 kV for 10 s, 15 kV as the separation voltage, 30 mmol/L phosphate buffer at pH 7.5 as running buffer and 5 mmoL/L Ru（bpy）3^2＋ in 50 mmol/L phosphate buffer at pH 7.5 in the detection cell） , the detection limit of disopyramide was 10 μmoL/L（S/N = 3 ）. The results of protein binding indicated that the binding of disopyramide with plasma protein was linear with a correlation coefficient of 0. 9999 when the ranges of concentration of disopyramide in plasma were 1.6 -8.2 retool/L, and the human plasma protein binding rate of disopyramide was 90.4%.