中文摘要：

有三在结构上的牛的浆液白朊(BSA ) 的相互作用联系了 isoflavonoids， genistein， puerarin 和 daidzein，被荧光在生理的条件下面学习分光镜的技术。当静态的熄灭，有 BSA 的这些混合物的熄灭的机制被建议，有约束力的常数基于熄灭结果的荧光在不同温度是坚定的。在领受人和 BSA 之间的精力和距离的转移效率根据 F 的机制被调查 ? rster 精力迁移。根据热力学的参数，代理力量主要是恐水病的力量，这被建议了。到 BSA 的三 isoflavonoids 的有约束力的力量的比较证明由 5-OH 和 8-Glc 的替换能提高有约束力的亲密关系。这些在工作获得了的所有能更好做我们理解行动的模式和 isoflavonoids 的药理学活动。

英文摘要：

The interactions of bovine serum albumin （BSA） with three structurally related isoflavonoids, genistein, puerarin and daidzein, were studied under physiological conditions by fluorescence spectroscopic technique. The quenching mechanism of these compounds with BSA was suggested as static quenching and the binding constants were determined at different temperatures based on the fluorescence quenching results. The transfer efficiency of energy and distance between the acceptor and BSA were investigated on the basis of the mechanism of the Forster energy transference. According to the thermodynamic parameters it has been suggested that the acting force be mainly hydrophobic force. The comparison of binding potency of the three isoflavonoids to BSA showed that the substitution by 5-OH and 8-Glc could enhance the binding affinity. All these obtained in the work can make us better understand the mode of the action and pharmacological activities of the isoflavonoids.