中文摘要：

目的 研究泻火补肾汤（XHBSD）对神经干细胞（NSCs）移植入脑出血大鼠脑室下区后血管细胞间黏附分子（VCAM～1）和转化生长因子（TGF—β1）基因表达的影响，探讨泻火补肾汤能否诱导免疫耐受。方法SD大鼠随机分为六A（正常组）5只，B（假手术组）、C（模型组）、D（模型治疗组）、E（移植组）、F（移植治疗组）各25只。用RT—PCR方法观察各组术后第2、4、7、14、28日TGF—β1．mRNA及VCAM-1mRNA的表达。结果B、C、D、E、F五组VCAM—1mRNA和TGF—β1．mRNA分别在第2、4日表达达高峰（P〈0．05）。VCAM—1mRNA在各时间点E组表达最强（P〈0．05），而TGF—1mRNAF组表达最强（P〈0．01）。同时间点除第28日外，F组比E组、D组比c组TGF—p．mRNA表达增强（P〈0．05），而VCAM-1mRNA表达减弱（P〈0．05）。结论泻火补。肾汤可通过促进TGF—β1 mRNA的表达，降低VCAM—1mRNA的表达，有助于诱导免疫耐受。

英文摘要：

Objective： To observe the effect fo Xiehuobushen Decoction（XHBSD） on the gene expressions of transforming growth factor -betal（TGF - β1 ）and vascular cell adhesion molecular - 1 （VCAM - 1 ）in the rats brain of NSCs - transplanted intracerebral hemorrhage, in order to investigate whether the XHBSD can induce immunotolerance or not. Methods： One hundred and thirty Sprague - Dawley Rats were randomly divided into six groups： A group （normal group）（ n =5）, B group （sham operation group）（ n =25）, C group （model group）（ n =25）, D group（treated model group） （ n = 25）, E group（transplanted group） （ n = 25）, F group（treated transplanted - group） （ n = 25）. The expressions ofTGF- β1 mRNA and VCAM - lmRNA were assayed by RT -PCR at 2d, 4d, 7d, 14d, 28d after the operation. Results： The expressions of VCAM -1mRNA and TGF - β1 mRNA in B, C, D, E, F groups arrived the peak at 2nd day and 4d respectively, The expression of VCAM - 1mRNA in E group was the highest among all the groups, while the expression of TGF - β1 mRNA in F group was the highest . At the same time except 28th day, the expression of TGF - β1 mRNA in F and D groups were significantly higher than E and C groups respectively, and the expression of VCAM - 1mRNA in F and D groups were lower than E and C groups respectively. Conclusion： XHBSD can promote the expression of TGF - β1 mRNA and inhibit the expression of VCAM - 1mRNA in the rats brain of NSCs - transplanted intracerebral hemorrhage, and may induce the immunotolerance of the receptor.