中文摘要：

目的探讨葛根芩连汤（GGQLD）对KK-Ay糖尿病小鼠血浆中脂多糖（LPS）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）的量及肠道菌群的调节作用。方法以普通饲料喂养的C57BL/6J小鼠作为对照组,ig等量蒸馏水。将高脂饲料喂养的KK-Ay小鼠分为5组,吡格列酮组和模型组分别ig给予盐酸吡格列酮5 mg/kg和等量蒸馏水,GGQLD高、中、低剂量组ig分别给予GGQLD生药40、13.3、4.44 g/kg。各组均连续给药4周后,以鲎试剂显色基质法测定血浆中LPS的量,用酶联免疫吸附试验法（ELISA）检测血浆中TNF-α及IL-6的水平,采用变性梯度凝胶电泳（DGGE）技术分析小鼠肠道菌群的结构。结果与模型组相比,GGQLD高、中剂量组均能显著降低小鼠血浆内LPS的量（P〈0.05）,分别降低了15.61%、14.48%;GGQLD高、中、低剂量组IL-6的量与模型组比较分别下降了56.86%、37.12%、30.21%,具有一定的剂量依赖性,且有统计学意义（P〈0.05）;GGQLD高、中、低剂量组TNF-α的量均明显低于模型组（P〈0.05）,分别下降了28.32%、30.70%、23.42%。GGQLD高剂量组DGGE图谱条带数目明显增多,且经克隆、测序和Blast比对分析,约翰逊氏乳杆菌Lactobacillus johnsonii为GGQLD高剂量组特有,说明GGQLD可明显调节肠道菌群结构。结论 GGQLD抗2型糖尿病胰岛素抵抗作用可能与其改善LPS、TNF-α、IL-6等炎症因子,及调节肠道菌群结构相关。

英文摘要：

Objective To explore the effect of Gegen Qinlian Decoction（GGQLD） on LPS, TNF-α, IL-6, and intestinal flora in diabetic KK-Ay mice. Methods C57BL/6J mice with ordinary feed were taken as the normal control group and orally administrated with equal distilled water. The KK-Ay mice fed with high-fat diet were divided into five groups： pioglitazone group, blank group（model group）, high, medium, and low dose GGQLD group, and orally administrated with pioglitazone hydrochloride（5 mg/kg）, distilled water, and GGQLD（crude drug 40, 13.3, and 4.44 g/kg）, respectively. The oral administration for six groups lasted for four weeks. Tumor necrosis factor（TNF-α）, interleukin 6（IL-6）, and endotoxin（LPS） levels in the plasma were determined by enzyme-linked immunosorbent assay（ELISA）; Gut microbial communities were assayed by polymerase chain reaction（PCR） and PCR-denaturing gradient gel electrophoresis（PCR-DGGE） methods. Results Compared with the model group, the LPS levels in the plasma of mice were significantly reduced by 15.61% and 14.48% respectively in the Gegenqinlian Decoction of high and medium dose group（P〈 0.05）, the IL-6 levels in plasma of mice were significantly reduced by 56.86%, 37.12% and 30.21% respectively in high, medium, and low dose GGQLD group（P〈 0.05）, and the TNF-α levels in plasma of mice were significantly reduced by 28.32%, 30.70%, and 23.42% respectively in high, medium, and low dose GGQLD group（P〈 0.05）. The number of DGGE bands in high dose group significantly increased, and by cloning, sequencing, and Blast analysis, Lactobacillus johnsonii only existed in the high dose group; The results showed that GGQLD could regulate the structure of intestinal flora in KK-Ay mice. Conclusion The mechanisms of anti-diabetic effects of GGQLD in type 2 diabetic KK-Ay mice are probably related with the anti-inflammation and regulation of intestinal flora.