中文摘要：

背景：羟基磷灰石与钛复合制备的复合材料是理想的人体骨替代材料。但纯钛金属基板由于无生物活性,难以在其表面生长羟基磷灰石,因此必须对钛片进行预处理。目的：制备二氧化钛纳米管/羟基磷灰石复合材料,并观察预钙化对羟基磷灰石沉积速率的影响。设计、时间及地点：对比观察实验,于2007-05/2008-01在华中师范大学物理科学与技术学院纳米技术中心完成。材料：钛片由宝鸡金属研究所提供,厚度250μm,纯度99.7%。方法：用电化学方法在钛片表面制得二氧化钛的纳米管,并通过热处理得到晶态的二氧化钛。将钛片先后浸泡在饱和氯化钙、磷酸二氢钾溶液中进行预钙化处理。然后在37℃条件下将已有二氧化钛阵列的钛片置于模拟体液中浸泡。主要观察指标：于浸泡1,3,5,7,14d后采用扫描电子显微镜观察样品表面形貌的显微结构,复合材料的结构采用X射线衍射仪进行表征。结果：扫描电镜结果显示：浸泡14d后,羟基磷灰石在预钙化处理钛片表面形成涂层,基本将纳米管表面覆盖;而未经预钙化处理钛片形成较少的羟基磷灰石,没有形成涂层。X射线衍射仪结果显示：浸泡14d后,经过预钙化处理的纳米管阵列表面出现较多的羟基磷灰石,可以清楚地观察到羟基磷灰石的各个特征峰。结论：利用仿生模拟法制备羟基磷灰石复合材料过程中,通过预钙化处理,羟基磷灰石的生长速率显著提高。

英文摘要：

BACKGROUND： Hydroxyapatite （HAP） titanium complex prepared composite is ideal human bone substitutes. However, pure titanium metal base without bioactivity is difficult to incubate HAP, and it is necessary to do pretreatment for titanium plate. OBJECTIVE： To prepare the composite of TiO2 nanotubes/HAP and to estimate the effect of pre-calcification procedure on the deposition rate of HAP on TiO2 nanotubes. DESIGN, TIME AND SETTING： A controlled observational experiment was performed at the Nano Center, College of Physical Science and Technology, Central China Normal University from May 2007 to January 2008. MATERIALS： Titanium plate was supplied by Baoji Mental Institute, China, with the depth of 250 μm and purity of 99.7%. METHODS： TiO2 nanotubes were gained through electrophoresis, and changed into the crystalline state TiO2 by heat treatment. Titanium plate was immersed in saturated calcium chloride and potassium dihydrogen phosphate for pre-calcification. Subsequently, TiO2 covered titanium plate was immersed in simulated body fluid at 37℃. MAIN OUTCOME MEASURES： At 1, 3, 5, 7, 14 days following immersion, microstructure of the sample surface was observed using scanning electron microscope. The structure of composite was analyzed utilizing X-ray diffractometer. RESULTS： Scanning electron microscope photographs showed that after immersed for 14 days, a large amount of HAP was deposited at the surface of the TiO2 nanotubes, which were pre-calcified, but there was little HAP on the samples, which were not pre-calcified. Results of X-ray diffractometer displayed that after immersed for 14 days, a large amount of HAP was deposited at the surface of the TiO2 nanotubes, which were pre-calcified, and the characteristic peak of HAP could be clearly observed. CONCLUSION： The rate of HAP deposition on TiO2 nanotubes was markedly improved by pre-calcification during HAP composite preparation using the bionic simulation method.