中文摘要：

采用透射电子显微镜研究了拟穴青蟹受精膜形成过程中线粒体的变化及其功能。结果显示,大量线粒体首先高度密集于即将发生胞吐的皮质中;随着胞吐的发生,线粒体也随之快速分散到皮层反应部位,线粒体通过分裂、出芽等方式而迅速增多,内质中的线粒体大量向皮质迁移,参与皮层反应;当受精膜开始举起时,线粒体多聚集在新质膜下,参与新质膜的修复和形成;受精膜完全举起后,皮质中的线粒体迅速减少。线粒体在形态、数量及分布等方面的变化,能够反映受精膜形成不同阶段生理活动的旺盛程度以及需能情况,并从细胞水平阐释受精膜形成机制。

英文摘要：

The changes and functions of mitochondria during the process of fertilization membrane（Fm）formation in Scylla paramamosain were investigated by transmission electron microscope.The results are as follows：Many mitochondria assembled densely at one place in the cortex just before the outburst of the ring-shaped granules.When exocytosis happened,numerous mitochondria scattered rapidly around the cortex where cortical reaction was proceeding.Mitochondria increased dramatically by splitting and budding,meanwhile lots of mitochondria that formed in endoplasm migrated to cortex,as numerous ring-shaped granules involving exocytosis needed abundant energy.While Fm was swelling,most of the mitochondria gathered under the new plasm membrane to participate in its restoring and formation.After Fm swelled completely,mitochondria decreased suddenly and disappeared since cortical granules＇ exocytosis had ceased.In short,the changes of mitochondria on its numbers,shapes and distribution,reflected the trait on physiological activation and energy supply in different stages of Fm formation.From the angle of the changes and functions of mitochondria,this study illuminated the mechanism of Fm formation on cellular level,and provided a new research field for Fm formation in Crustacea.