中文摘要：

目的对快速酶联免疫吸附试验（ELISA）的血吸虫病诊断实验条件进行优化,并观察其应用价值。方法同时将抗原、待测血清、HRP-SPA共置进行反应,替代常规ELISA法的分步反应。通过比较不同稀释度HRP-SPA的检测效果,确定其最佳工作浓度。观察待测血清和HRP-SPA不同混合反应时间对检测效果的影响,优化快速ELISA法的检测条件。用快速ELISA法、常规ELISA法和胶体染料试纸条法（DDIA）平行检测慢性血吸虫病人、华支睾吸虫病人及健康人血清,观察快速ELISA法的血吸虫病诊断实用价值。结果 HRP-SPA的最佳工作浓度为1∶2 000,待测血清和HRP-SPA最佳混合反应条件是37℃同步反应60 min。快速ELISA法检测慢性血吸虫病人血清抗体阳性检出率为93.8%,与华支睾吸虫病人血清的交叉反应率为4.0%,检测健康人血清的假阳性率为1.5%,与常规ELISA法和DDIA检测结果相似。结论快速ELISA法具有快速、简便等优点,是一种简便实用的血吸虫病免疫诊断方法。

英文摘要：

Objective To optimize the experimental conditions of fast enzyme-linked immunosorbent assay（F-ELISA） and evaluate its performance for immunodiagnosis of schistosomiasis japonica.Methods HRP-SPA was used at different concentrations in the assay to determine the best HRP-SPA concentration by comparing the detection accuracy.The working conditions of F-ELISA were optimized by examining the ratio of absorbance values of positive and negative controls with different incubation time of serum samples and HRP-SPA.To evaluate the performance of F-ELISA in diagnosis of schistosomiasis,the detection accuracy of F-ELISA was compared with routine ELISA and dipstick dye immunoassay（DDIA） by testing serum samples from both healthy subjects and those clinically diagnosed with schistosomiasis and clonorchiasis in parallel.Results In F-ELISA,the best working concentration of HRP-SPA was 1∶2 000 while the optimized incubation condition for serum samples and HRP-SPA was 60 min at 37 ℃.Using F-ELISA,we identified 93.8% to be schistosomiasis positive among chronic schistosomiasis subjects,4% within the clonorchiasis group,and a false positive rate of 1.5% in healthy individuals.This diagnostic accuracy for F-ELISA was similar to routine ELISA and DDIA.Conclusions F-ELISA combines the 2-steps of routine ELISA into a single step making the assay process faster and simpler without sacrificing the diagnostic accuracy.With further investigations,F-ELISA as an easy and practical method is promising to be applied for schistosomiasis diagnosis in the field.