中文摘要：

5 氟尿嘧啶(5-FU ) 举办许多反肿瘤活动，广泛地适用于癌症的治疗。然而，由于它的狭窄的治疗学的索引在临床的实践决定 5-FU 的血浆集中是必要的。因此，一个简单、经济、敏感的高效的液体层析(HPLC ) 方法在人的血浆为 5-FU 的决心被开发并且验证。乙醇醋酸盐被选择为抽取试剂。色析法的分离在 Diamonsil C18 列上被执行(250 公里獲汥? 楷桴琠敨愠獤牯瑰潩 ? 慲楴? 楷桴畯 ?敭桴湡汯

英文摘要：

5-Fluorouracil （5-FU） has a broad spectrum of anti-tumor activity, widely applied to the treatment of can- cers. However, it is necessary to determine the plasma concentration of 5-FU in clinical practice due to its narrow therapeutic index. Therefore, a simple, economic and sensitive high-performance liquid chromatography （HPLC） method was developed and validated for the determination of 5-FU in human plasma. Ethyl acetate was chosen as ex- traction reagent. Chromatographic separation was performed on a Diamonsil C18 column （250 mm × 4.6 mm i.d., 5 μm） with the mobile phase consisting of methanol and 20 mmol/L ammonium formate using a linear gradient elution at a flow rate of 0.8 mL/min. 5-FU and 5-bromouracil （5-BU） were detected by UV detector at 265 nm. The calibra- tion curve was linear over the concentration range of 5--500 ng/mL and the correlation coefficient was not less than 0.992 6 for all calibration curves. The intra- and inter-day precisions were less than 10.5%, and 4.3%, respectively, and the accuracy was within ＋3.7%. The recovery at all concentration levels was 80.1-8.6%. 5-FU was stable under possi- ble conditions of storing and handling. This method is proved applicable to therapeutic drug monitoring and pharma- cokinetic studies of 5-FU in human.