目的:同时测定不同产地杏香兔耳风药材中主要酚酸类成分的含量,为完善杏香兔耳风药材质量标准和药材道地产地选择提供参考依据。方法:采用RP-HPLC法同时测定杏香兔耳风药材中主要的两类酚酸成分的含量,即单咖啡酰奎宁酸类(caffeoylquinic acids,CQA)和二咖啡酰奎宁酸类(dicaffeoylquinic acids,DCQA)。色谱柱:Cosmosil RP-C18柱(250 mm×4.6 mm,5μm);流动相:乙腈-0.1%甲酸溶液,梯度洗脱;流速:1.0 mL/min;检测波长:328 nm;柱温:35℃。结果:不同产地杏香兔耳风中单咖啡酰奎宁酸和二咖啡酰奎宁酸的含量参差不齐;江西婺源、九江、上饶的杏香兔耳风中各酚酸的含量明显高于其他产地,广西地区的杏香兔耳风药材酚酸类含量最低;绿原酸和3,5-DCQA是杏香兔耳风药材中的主要酚酸成分。结论:本方法准确,简便,重复性良好,可用于不同产地杏香兔耳风药材酚酸类有效成分的检测;结果提示江西产杏香兔耳风为优势特色药材。
Objective:The contents of major phenolic acids in Ainsliaea fragrans from different habitats were determined,in order to improve quality standards of Ainsliaea fragrans and provide reference for optimization its daodi habitat. Methods:Separation and determination of two kinds of phenolic acids,caffeoylquinic acids( CQA) and dicaffeoylquinic acid( DCQA),in Ainsliaea fragrans were carried out by RP-HPLC with Cosmosil RP-C18column(250 mm × 4. 6 mm,5 μm),acetonitrile-0. 1% formic acid solution as mobile phase at the flow rate of 1. 0 mL /min,detection wavelength at 328 nm and column temperature at 35 ℃. Results:CQA and DCQA were uneven in Ainsliaea fragrans from different habitats. In the sample from Wuyuan,Jiujiang and Shangrao in Jiangxi,the contents of phenolic acids were higher than those from other habitats significantly. The sample from Guangxi habitat had the lowest phenolic acids content. And the chlorogenic acid and 3,5-DCQAs' percentage were higher than other phenolic acids. Conclusion:This method is accurate and easy with good repeatability,which can be used in determination of eight phenolic acids in Ainsliaea fragrans from different habitats. Ainsliaea fragrans in Jiangxi Province has good qulity.