中文摘要：

在脊椎动物胚胎开始期间， presomitic 中层房间进入一个部分程序产生体节，进程称为 somitogenesis。Mespo， bHLH 抄写因素家庭，的一个成员在这个过程起重要作用。然而， Mespo 表示怎么被调整，仍然保持不清楚。探讨这个问题，我们孤立在 Xenopus 包含 Mespo 5' flanking 区域的 4317 bp 的一个 genomic DNA 序列。酶试金证明这个在上游的序列举办抄写活动。这 genomic contig 是足够的从胚囊概括内长的 Mespo 的动态阶段特定、织物特定的表示模式到尾巴芽阶段的转基因的试金表演。我们进一步印射对 retinoic 酸发信号作出回应的 326 bp DNA 顺序。这些结果使清楚些 Mespo 表示怎么被调整，并且建议 retinoic 酸发信号小径通过调整 Mespo 在 somitogenesis 起作用。

英文摘要：

During vertebrate embryogenesis, presomitic mesoderm cells enter a segmental program to generate somite, a process termed somitogenesis. Mespo, a member of the bHLH transcription factor family, plays important roles in this process. However, how Mespo expression is regulated remains unclear. To address this question, we isolated a genomic DNA sequence containing 4317 bp of Mespo 5＇ flanking region in Xenopus. Luciferase assays show that this upstream sequence has transcription activity. Transgenic assay shows that this genomic contig is sufficient to recapitulate the dynamic stage- and tissue-specific expression pattern of endogenous Mespo from the gastrula to the tailbud stage. We further mapped a 326 bp DNA sequence responding to retinoic acid signaling. These results shed light on how Mespo expression is regulated, and suggest that retinoic acid signaling pathways play roles in somitogenesis through regulating Mespo.