本文提供了一种在聚二甲基硅氧烷(PDMS)基底上制备高密度蛋白微阵列的新方法。此方法的特点在于通过在PDMS基底上物理吸附乙二胺四乙酸二钠(EDTA)达到提高蛋白质分子转印效率的目的。AFIM检测结果表明:转印后的图案结构规整,边缘清晰。测得的蛋白条纹平均宽度约为500nm,条纹间距约为1μm,是一种高密度的蛋白微阵列。
In this paper, a new and facile route was developed to efficiently print protein microarrays onto hydrophobic polydimethylsiloxane (PDMS) substrate. The unique feature of this method was that protein could be easily transferred onto PDMS substrate by first physically adsorbing EDTA molecules on the PDMS surface. AFM results showed that the transferred patterns are of high fidelity and density with average width of about 500 nm and the interval of 1μm.