中文摘要：

客观: 由自体同源的移植和骨头髓的承诺的区别恢复心脏的自治的步功能间充质的干细胞，并且为病了的湾穴症候群的处理探索这种技术。方法: 从犬的骨头髓孤立的间充质的干细胞在由 5-azacytidine 的 vitro 膨胀文化、区分。在 canines 的病了的湾穴症候群的模型被与无线电频率技术切除湾穴节点建立。BrdU 标记的区分的间充质的干细胞自体同源地通过直接注射被移植进湾穴节点区域。在病了的湾穴症候群模型的心脏的自治的步功能上的间充质的干细胞的自体同源的移植的效果被 electrocardiography 评估，病理学并且 immunohistochemical 染色 technique.Results : 当区分的间充质的干细胞是进湾穴节点区域的 auto-transplanted 时，病了的湾穴症候群动物模型的步功能上有不同改进。在湾穴节点区域移植的间充质的干细胞在 vivo 被区分进类似的湾穴节点房间和 endothelial 房间，并且与本国的 cardiomyocytes 建立了差距连接。结论: 间充质的干细胞移植了进湾穴节点区域的承诺导致能区分进类似的湾穴节点房间并且在犬的病了的湾穴症候群模型改进步功能。

英文摘要：

Objective：To restore cardiac autonomic pace function by autologous trans- plantation and committed differentiation of hone marrow mesenchymal stern cells, and explore the technique for the treatment of sick sinus syndrome. Methods ： Mesenchymal stern cells isolated from canine bone marrow were culture-expanded and differentiated in vitro by 5-azacytidine. The models of sick sinus syndrome in canines were established by ablating sinus node with radio-frequency technique. Differentiated mesenchymal stem cells labeled by BrdU were autologously transplanted into sinus node area through direct injection. The effects of autologous transplantation of mesenchymal stern cells on cardiac autonomic pace function in sick sinus syndrome models were evaluated by electrocardiography, pathologic and immunohistochemical staining technique. Results：There was distinct improvement on pace function of sick sinus syndrome animal models while differentiated mesenchymal stem cells were auto-transplanted into sinus node area. Mesenchymal stern cells transplanted in sinus node area were differentiated into similar sinus node cells and endothelial cells in vivo, and established gap junction with native cardiomyocytes. Conclusion ： The committed- induced mesenchymal stern cells transplanted into sinus node area can differentiate into a- nalogous sinus node cells and improve pace function in canine sick sinus syndrome models.