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生物核磁共振波谱学
  • 项目名称：生物核磁共振波谱学
  • 项目类别：创新研究群体科学基金
  • 批准号：21221064
  • 申请代码：B0504
  • 项目来源：国家自然科学基金
  • 研究期限：2013-01-01-2015-12-31
  • 项目负责人：刘买利
  • 负责人职称：研究员
  • 依托单位：中国科学院武汉物理与数学研究所
  • 批准年度：2012
中文摘要：

本群体是在二十多年的发展过程中自然形成。群体成员以生物磁共振波谱分析和成像分析为核心，在磁共振基础、分析方法和应用研究方面做出了一系列创新性成果，为相关领域的发展做出了贡献，已经成了自己鲜明的特色和优势。我们拟充分利用并发挥群体成员多年来群在磁共振波谱分析和成像分析研究方面的优势和工作积累，以脑功能、脑疾病的物质基础为研究对象，针对现有研究方法的缺点和局限性，发展具有创新性的磁共振波谱与成像方法，为脑科学研究提供技术支撑；本群体还将应用所发展的磁共振方法，从结构生物学、神经化学、神经生理学的角度，在分子、组织和活体等不同水平上研究脑功能、脑疾病的物质基础。

中文主题词： 高场磁共振波谱仪；超极化129Xe肺部成像仪；生物磁共振分析；生命分析；多学科交叉
英文摘要：

High field magnetic resonance spectrometer；Hyperpolarization 129xe lung imaging spectrometer；Biological magnetic resonance (NMR) analysis；bio-analytical；multidisciplinary

英文主题词： High field magnetic resonance spectrometer；Hyperpolarization 129xe lung imaging spectrometer；Biological magnetic resonance (NMR) analysis；bio-analytical；multidisciplinary
结论摘要：

“生物核磁共振波谱学”国家创新群体主要围绕重要的科学问题(特别是生命科学中的科学问题)，开展核磁共振分析原理、技术、方法的创新研究；培养多学科交叉的人才；为相关领域的研究提供支撑；推动核磁共振学科的发展，引领我国多学科交叉的磁共振基础与应用研究。六年来，在国家自然科学基金委的资助下，群体成员围绕预期目标开展深入系统的合作研究，取得的主要成果包括1）研制成功具有自主知识产权的高场磁共振波谱仪并实现工程化及应用，获湖北省2013年度技术发明一等奖，“实现了我国高端谱仪从无到有的突破”；成功研制超极化129Xe肺部成像仪，实现了对肺部疾病模型动物的肺功能的定性定量分析；成功研制了动态核极化-核磁共振分子影像装置，实现了MRI分子影像的灵敏度增强。2）发展了快速、高灵敏的生物磁共振分析新方法与新技术，包括（1）用于原子分辨蛋白质结构解析的快速、高灵敏核磁共振技术；（2）用于高时空分辨蛋白质相互作用研究的核磁共振技术；（3）基于核磁共振及联用技术的代谢组分析新方法；（4）用于活体分析的多模态脑成像/活体波谱新方法。3）开展了磁共振方法与技术在生命分析中应用研究，包括膜相关蛋白高分辨三维结构的核磁共振解析、蛋白质间弱相互作用实验探测、重大疾病的发生发展机制的代谢组学研究，以及成瘾类疾病机理的脑功能成像及波谱研究。4）培养和引进了一批青年骨干，新增国家杰出青年科学基金获得者2人，973项目首席科学家1人，中组部万人计划青年拔尖人才2人，优青1人，青年千人1人，百人4人。群体学科背景和研究方向(物理/谱仪技术，生物磁共振/蛋白质动态过程、膜蛋白结构和活细胞磁共振)进一步拓展和提升，队伍知识结构和年龄分布更趋合理。近六年共发表SCI论文429篇，平均被引次数9.4次，后3年发表SCI论文272篇。其中包括Science 1篇，PNAS2篇，JACS13篇，Angew. Chem. Int. Ed. 9篇，Chem. Comm. 9篇，Chem. Eu. J. 7篇，J. Magn. Reson13篇，J.Proteome. Res.31篇，J. Phys. Chem. A/B/C31篇。群体核心成员合作发表的论文占27%。论文数和篇均引用数与美国国家强磁场实验室同期(2010.01-至今)发表的核磁共振论文接近(443篇，引用9.96次/篇)。

成果综合统计
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  • 期刊论文
  • 会议论文
  • 专利
  • 获奖
  • 著作
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期刊论文
Quasiclassical trajectory studies of the O(3P) + CX4(vk = 0, 1) --> OXv + CX3(n1n2n3n4) [X =
Multiple univariate data analysis reveals the inulin effects on the high-fat-diet induced metab
A N-15 CPMG relaxation dispersion experiment more resistant to resonance offset and pulse imperfecti
The toxicity of acute exposure to T-2 toxin evaluated by the metabonomics technique
Beryllium Fluoride Exchange Rate Accelerated by Mg2+ as Discovered by F-19 NMR
Observation of H-1-C-13 and H-1-H-1 proximities in a paramagnetic solid by NMR at high magnetic fiel
Ultrasmall Manganese Ferrite Nanoparticles as Positive Contrast Agent for Magnetic Resonance Imaging
NMR Based Cerebrum Metabonomic Analysis Reveals Simultaneous Interconnected Changes during Chick Emb
Dynamic changes in metabolic profiles of rats subchronically exposed to mequindox
Recent advances in protein NMR spectroscopy and their implications in protein therapeutics research
Rational design of hyperpolarized xenon NMR molecular sensor for the selective and sensitive determi
Bipolar pi-conjugation interrupted host polymers by metal-free superacid-catalyzed polymerization fo
Theoretical prediction of the linear isomers for rare gas-carbon disulfide complexes: He-CS2, Ne-CS2
Acute toxicity of zinc oxide nanoparticles to the rat olfactory system after intranasal instillation
Hydrothermal synthesis method of 5-(4 '-methylbiphenyl-2-yl)-1H-tetrazole
Heavy smokers show abnormal microstructural integrity in the anterior corpus callosum: A diffusion t
Quasiclassical Trajectory Studies of the O(P-3)+CX4(v(k)=0, 1) -> OX(v)+CX3(n(1)n(2)n(3)n(4))
Protein dynamics elucidated by NMR technique
Deficits of peripheral olfactory inputs reduce cell proliferation in the adult subventricular andsub
Multiple Univariate Data Analysis Reveals the Inulin Effects on the High-Fat-Diet Induced Metabolic
Diffusion tensor magnetic resonance imaging reveals visual pathway damage that correlates with clini
Nine-dimensional quantum dynamics study of the H-2 + NH2 -> H + NH3 reaction: a rigorous test of
The PHD1 finger of KDM5B recognizes unmodified H3K4 during the demethylation of histone H3K4me2/3 by
Decreased coherence between the two olfactory bulbs in Alzheimer's disease model mice
High-Fat Diet Induces Dynamic Metabolic Alterations in Multiple Biological Matrices of Rats
Metabolic Influence of Acute Cyadox Exposure on Kunming Mice
Amyloid-beta Deposition and Olfactory Dysfunction in an Alzheimer's Disease Model
Anatomical MRI templates of tree shrew brain for volumetric analysis and voxel-based morphometry
Lateral Entorhinal Modulation of Piriform Cortical Activity and Fine Odor Discrimination
Gut Microbiota Composition Modifies Fecal Metabolic Profiles in Mice
Iterative Cross-Correlation Analysis of Resting State Functional Magnetic Resonance Imaging Data
A Robust Automatic Phase Correction Method for Signal Dense Spectra
Systemic Responses of Mice to Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis Using H-1 NMR
Time-Dependent Wave Packet Study of the H2 + CH3 --> H + CH4 Reaction.
In-Channel and In-Plane Li Ion Diffusions in the Superionic Conductor Li10GeP2S12 Probed by Solid-St
Targeted In Vivo Imaging of Microscopic Tumors with Ferritin- based Nanoprobes Across Biological Bar
Visualizing an Ultra-Weak Protein-Protein Interaction in Phosphorylation Signaling
Subtle Dynamics of holo Glutamine Binding Protein Revealed with a Rigid Paramagnetic Probe
Solid-State NMR Shows That Dynamically Different Domains of Membrane Proteins Have Different Hydrati
Asymmetric simultaneous phase-inversion cross-polarization in solid-state MAS NMR: Relaxing selectiv
Highly sensitive detection of mercury (II) in aqueous media by tetraphenylporphyrin with a metal ion
Strategies for Protein NMR in Escherichia coli
A comparison study of the H + CH4 and H + SiH4 reactions with eight-dimensional quantum dynamics: no
Charge-transfer-featured materials-promising hosts for fabrication of efficient OLEDs through triple
Effects of reactant rotation on the dynamics of the OH + CH4 -> H2O + CH3 reaction: A six-dimensi
H-1 NMR metabolomics study of metastatic melanoma in C57BL/6J mouse spleen
Impact of Magnesium(II) on Beryllium Fluorides in Solutions Studied by F-19 NMR Spectroscopy
NMR structures of fusion peptide from influenza hemagglutinin H3 subtype and its mutants
pH- and concentration-induced micelle-to-vesicle transitions in pyrrolidone-based Gemini surfactants
Systemic Responses of BALB/c Mice to Salmonella typhimurium Infection
New Insight into the Hydrocarbon-Pool Chemistry of the Methanol-to-Olefins Conversion over Zeolite H
Host-Guest Interactions in Dealuminated HY Zeolite Probed by C-13-Al-27 Solid-State NMR Spectroscopy
Efficient p-Conjugated Interrupted Host Polymer by Metal-Free Polymerization for Blue/Green Phosphor
Highly mobile segments in crystalline poly(ethylene oxide)(8): NaPF6 electrolytes studied by solid-s
Peripheral Input-dependent Structural Plasticity of Granule Cells in The Mouse Olfactory Bulb
Mode specific dynamics of the H2 + CH3 --> H + CH4 reaction studied using quasi-classical tr
Direct Observation of Ca2+-Induced Calmodulin Conformational Transitions in Intact Xenopus laevis Oo
Altered spontaneous brain activity in heavy smokers revealed by regional homogeneity
MRI-visible liposome nanovehicles for potential tumor-targeted delivery of multimodal therapies
Labeling Strategy and Signal Broadening Mechanism of Protein NMR Spectroscopy in Xenopus laevis Oocy
Population transfer HMQC for half-integer quadrupolar nuclei
Integrative Transcriptomic and Metabonomic Molecular Profiling of Colonic Mucosal Biopsies Indicates
Phase Transition and Preferential Alcohol Adsorption of Poly(N,N-diethylacrylamide) Gel in Water/Alc
Slight channel difference influences the reaction pathway of methanol-to-olefins conversion over aci
Body temperature sensitive micelles for MRI enhancement
Developmental Changes for the Hemolymph Metabolome of Silkworm (Bombyx mori L.)
Paramagnetic relaxation enhancement solid-state NMR studies of heterogeneous catalytic reaction over
Recent advances in solid state NMR characterization of zeolites
An optimized method for NMR-based plant seed metabolomic analysis with maximized polar metabolite ex
Rotational mode specificity in the Cl + CHD3 -> HCl + CD3 reaction
Ca2+ modulating alpha-synuclein membrane transient interactions revealed by solution NMR spectroscop
pH-Triggered Au-fluorescent mesoporous silica nanoparticles for F-19 MR/fluorescent multimodal cance
Systemic responses of mice to dextran sulfate sodium-induced acute ulcerative colitis using 1H
High-fat diet induces dynamic metabolic alterations in multiple biological matrices of rats.
White Matter Development is Potentially Influenced in Adolescents with Vertically Transmitted H
An eight-dimensional quantum dynamics study of the Cl + CH4--> HCl + CH3 reaction.
Experimental Evidence on the Formation of Ethene through Carbocations in Methanol Conversion ov
A europium-lipoprotein nanocomposite for highly-sensitive MR-fluorescence multimodal imaging
Region-specific cerebral metabolic alterations in streptozotocin-induced type 1 diabetic rats:
Molecular Dynamics Simulations of the Escherichia coli HPPK Apo-enzyme Reveal a Network of Conf
Metabolic Phenotypes Associated with High-Temperature Tolerance of Porphyra haitanensis Strains
Room temperature stable zinc carbonyl complex formed in zeolite ZSM-5 and its hydrogenation reactivi
Altered brain functional networks in heavy smokers
The metabolic responses to hepatitis B virus infection shed new light on pathogenesis and targets fo
Metabolic influence of acute cyadox exposure on Kunming mice.
Theoretical studies for the N2-N2O van der Waals complex: The potential energy surface, intermo
绿豆（Vigna Radiata）代谢物组成的核磁共振定量分析
大鼠尿液中N-氧化烟酰胺和伪尿嘧啶核苷的NMR分析
吲哚丙酮酸的酮-烯醇互变异构化的NMR研究
乙酰化修饰抑制α-synuclein的纤维化聚集（英文）
铜丝光沸石上甲醇羰基化反应的固体核磁共振研究
Characterization and Comparison of Commercial Chinese Cereal and European Grape Vinegars Using ^1H NMR Spectroscopy Combined with Multivariate Analysis
固体酸催化剂结构与催化反应机理的核磁共振研究（英文）
固体核磁共振研究分子筛的新进展
涡虫代谢物组成的核磁共振分析
Hydrophobic terminal group of surfactant initiating micellization as revealed by ^1H NMR spectroscopy
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