欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
位置：立项数据库 > 立项详情页
中文摘要：
精制和纯化天然产物的难点在于杂质与产物的物化性质十分相近。课题组以EGCG葛根素、溶菌酶等为研究对象，研究了环糊精键合介质、整体柱分离技术、超分子介质的制备及其应用、分子印迹超分子分离技术等基于弱相互作用和超分子识别的生物活性分子分离新技术，一步分离纯化的纯度大于98％以上。建立了一套基于膜乳化的均一可控微球的介质制备方法，完成按需设计配基密度和介质孔径的制备介质，以及生物大分子配基的亲和色谱分离新方法。这些研究和方法提高了色谱分离效率，简化和集成了分离纯化过程。在此基础上，建立了乙肝免疫球蛋白等天然产物分离纯化平台和示范工艺。进行了EGCG、葛根素等和环糊精分子模拟及其形成超分子结构的分子识别机理研究。研究了溶菌酶、乙肝病毒表面抗原等在介质表的吸附与变构机理。为提升我国天然活性成分分离纯化技术水平和中药现代化提供新方法和基础数据。发表论文100余篇，其中SCI文章97篇；申报国家发明专利15项，其中授权7项；获得国家发明二等2项，省部级二等奖1项，出版专著3部；1人次入选教育部新纪优秀人才，1人次入选北京市科技新星计划，培养博士研究生13人，硕士研究生20人。
结论摘要：
英文主题词natural product, separation and purification;adsorption; structural transformation on interface; molecular recgonition
成果综合统计
期刊论文
会议论文
专利
获奖
著作
期刊论文
Glycerol-Assisted Hydrophobic Interaction Chromatography Improving Refolding of Recombinant Human Gr
Purification of Glutathione by Immobilized Metal Ion Affinity Chromatography with Thiourea as the Li
PEGylation markedly enhances the in vivo potency of recombinant human non-glycosylated erythropoieti
Site-specific PEGylation of recombinant human non-glycosylated erythropoietin and characterization o
One-step puri?cation of YLLIP2 isoforms from Candida sp. 99–125 by polyethyleneimine modi?ed poly(gl
Separation and puri?cation of hydrolysable tannin from Geranium wilfordii Maxim by reversed-phase an
Nano?brous scaffold from self-assembly of β-sheet peptides containing phenylalanine for controlled r
Ef?cient separation of homologousα-lactalbumin from transgenic bovine milk using optimized hydrophob
A Novel Medium Poly(vinyl acetate-triallyl isocyanurate-divinylbenzene) Coupled with Oligo-β-Cyclode
Regulation of protein multipoint adsorption on ion-exchange adsorbent and its application to the pur
Investigation of the Inclusions of Puerarin and Daidzin withβ-Cyclodextrin by Molecular Dynamics Sim
Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interact
Relationship between thermal inactivation and conformational change of Yarrowia lipolytica lipase an
Salt-, pH- and temperature-responsive semi-interpenetrating polymer network hydrogel based on poly(a
Functional dependency of structures of ionic liquids: do substituents govern the selectivity of enzy
Mixed-mode retention mechanism for (-)-epigallocatechin gallate on a 12% cross-linked agarose gel me
On-column refolding of consensus interferon at high concentration with guanidine-hydrochloride and p
Poly(aspartic acid) Super-Absorbent Resin Produced by Chemical Crosslinking and Physical Freeze/Thaw
Immobilized Triton X-100-assisted refolding of Green Fluorescent Protein-Tobacco Etch Virus protease
Controlled Release and Entrapment of Enantiomers in Self-Assembling Scaffolds Composed of beta-Sheet
Modeling and prediction of the mixed-mode retention mechanisms for puerarin and its analogues on n-o
Thermodynamic and NMR investigations on the adsorptionmechanismof puerarin with oligo-β-cyclodextrin
Controlling Degradation and Physical Properties of Chemical Sand Fixing Agent-Poly(aspartic acid) by
Effects of alcohol and solvent on the performance of lipase from Candida sp. in enantioselective est
Process utilized oligo-β-cyclodextrin substituted agarose gel medium for ef?cient puri?cation of pac
High-level expression of extracellular lipase Lip2 from Yarrowia lipolytica in Pichia pastoris and i
Preparation and characterization of polyethyleneimine modified ion-exchanger based on poly (methacry
NMR Studies on the interaction between (-)-epigallocatechin gallate and cyclodextrins, free and bond
One-step simultaneous purification of three water-soluble constituents in crude extracts from Danshe
Study of the properties of molecularly imprinted polymers by computational and conformational analys
Selective recognition and large enrichment of dimethoate from tea leaves by molecularly imprinted po
Preparation and characterization of a novel pH-, thermo-, and ionic strength-responsive hydrogels ba
A highly ef?cient hydrophobic interaction chromatographic absorbent improved the puri?cation of hepa
Analysis of the Conformational Stability and Activity of Candida antarctica Lipase B in Organic Solv
A highly e?cient integrated chromatographic procedure for the puri?cation of recombinant hepatitis B
Application of surface molecular imprinting adsorbent in expanded bed for the adsorption of Ni2+ and
The influence of trace TiO2 on adsorption of Ag+-imprinted adsorbents made from chitosan and myceliu
One-step rapid determination and purification of puerarin from Radix puerariae by n-octylamine-modif
Enhancement of the isolation selectivity of isoflavonoid puerarin using oligo-beta-cyclodextrin coup
Controlling Factors on Droplets Uniformity in Membrane Emulsification: Experiment and Modeling Analy
Synthesis and characterization of a novel soil stabilizer based on biodegradable poly(aspartic acid)
Different Effects of L-Arginine on Protein Refolding: Suppressing Aggregates of Hydrophobic Interact
Preparation of Uniformly Sized Agarose Microcapsules by embrane Emulsification for Application in So
Controlled release and interaction of protein using self-assembling peptide RATEA16 nanofiber hydrog
Antimicrobial activities of hydrophilic polyurethane/titanium dioxide complex film under visible lig
相关项目
谭天伟的项目