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中文摘要：
发展灵敏快速简便的分析方法检测DNA损伤，对于疾病早期诊断、环境保护、药物筛选、以及化合物毒性检测等都具有重要意义。本项目利用层层组装技术将DNA固定在电极表面，建立了新的检测DNA损伤的电化学方法。（1）采用带负电荷的电活性指示剂，由于其与DNA的特异性相互作用，其电化学响应对未损伤和被损伤的DNA表现出明显区别。（2）利用某些酶对DNA的解链作用可以放大检测DNA损伤的电化学信号，更灵敏地检测DNA损伤。（3）建立了简单方便的体外电化学方法，成功地模拟了在生命体内DNA的损伤过程和其它过程。（4）充分利用层层组装方法的优势，不但将天然DNA固定在电极表面，而且通过选择和控制组装的不同组分可以实现修饰电极的各种功能。三年来，发表SCI论文30篇；已有3名博士、6名硕士研究生毕业；项目主持人2010年曾赴美国University of Connecticut与James F. Rusling教授进行短期合作研究。
结论摘要：
英文主题词DNA damage; Layer-by-layer assembly; Enzymatic reactions; Electrochemical detection.
成果综合统计
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期刊论文
Comparative bioelectrochemical study of two types of myoglobin layer-by-layer films with alumina: Va
Loading behavior of {chitosan/hyaluronic acid}(n) layer-by-layer assembly films toward myoglobin: An
Interaction between myoglobin and hyaluronic acid in their layer-by-layer assembly: Quartz crystal m
Layer-by-layer assembly of myoglobin and nonionic poly(ethylene glycol) through ion-dipole interacti
pH-dependent behaviors of electroactive myoglobin loaded into layer-by-layer films assembled with al
Study on direct Electrochemistry of glucose oxidase stabilized by cross-linking and immobilized in s
Loading/release behavior of (chitosan/DNA)(n) layer-by-layer films toward negatively charged anthraq
Assembly of myoglobin layer-by-layer films with poly(propyleneimine) dendrimer-stabilized gold nanop
Layer-by-layer films of hemoglobin or myoglobin assembled with zeolite particles: Electrochemistry a
Assembly of layer-by-layer films of electroactive hemoglobin and surfactant didodecyldimethylammoniu
Salt-induced swelling and electrochemical property change of hyaluronic acid/myoglobin multilayer fi
Electrochemically and catalytically active layer-by-layer films of myoglobin with zirconia formed by
Cyclic voltammetric detection of chemical DNA damage induced by styrene oxide in natural dsDNA layer
Conductive effect of gold nanoparticles encapsulated inside polyamidoamine (PAMAM) dendrimers on ele
Interaction of myoglobin with poly(methacrylic acid) at different pH in their layer-by-layer assembl
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