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中文摘要：
本项目将围绕植物激素超微量检测以及原位探测开展研究。针对植物激素超微量分析的关键技术及环节，拟通过微型化样品制备技术、毛细管色谱固定相和分离模式、质谱探针等研究，建立拟南芥和水稻等植物中多种植物激素的快速、灵敏、高通量的色谱－质谱联用分析平台，为我国植物学家的相关研究，提供稳定可靠的植物激素测定数据；同时拟采用微电极/阵列技术，通过进一步提高电化学检测的灵敏度、选择性以及稳定性，在植物活体、组织微区及单个植物细胞水平建立具有电活性植物激素分子的原位、瞬时、动态、并行监测方法，力争在植物激素的原位监测方面有所突破。项目的实施可望为植物激素研究提供急需的、切实可行的分析技术和方法，而且也将促进分析化学与植物科学交叉学科研究发展。
英文摘要：
Plant hormones；Ultra-sensitive detection；HPLC-MS；In-situ monitoring；
结论摘要：
内源性植物激素是重要的微量的小分子物质，调控着植物生长发育的每一个过程。因此，开发高通量、高灵敏度和高选择性的分析方法对于研究植物激素的生物功能等具有十分重要的意义。本项目围绕植物激素超微量检测以及原位探测两个方面开展研究工作。在植物激素超微量检测方面，我们以液相色谱-质谱技术为基本手段，重点探索了植物激素的样品前处理方法，如整体柱微萃取、磁固相萃取、化学衍生等。在此基础上建立了植物组织中细胞分裂素和酸性植物激素的若干液相色谱-质谱分析方法，如建立的整体柱微萃取-液相色谱-质谱在线联用分析方法，可以实现20mg鲜重植物样品中细胞分裂素和5mg鲜重样品中酸性植物激素的测定；发展的磁固相萃取-原位衍生技术与液相色谱-质谱联用方法，简化了植物样品的前处理过程，提高了分析通量。在植物激素原位探测方面，我们主要致力于发展微型化的电化学探针技术，建立了若干种植物激素等信号分子的实时检测方法，如研制了竖直排列的TiC@C/Pt核壳结构纳米纤维阵列电极（TiC@C/Pt-QANFAS），实现了单细胞水平生长素胞吐释放的实时检测；利用石墨微电极，金属圆盘微电极阵列，真空溅射镀金拉制毛细管，制备了可在体实时检测单个目标分子或同时在体实时检测多个目标分子的电化学探针，并在紫外胁迫和干旱胁迫的条件下，连续监测植物体内一氧化氮，活性氧和pH的水平随时间变化状况。该项目所建立的植物激素的超微量检测方法已经为植物学家提供了大量的植物激素分析数据；所建立的植物激素的实时检测方法有望为应用于植物激素的实时动态监测以及相关机制研究。
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期刊论文
Label-Free Quantitative Proteomic Analysis of Abscisic Acid Effect in Early-Stage Soybean under Floo
CYTOKININ OXIDASE/DEHYDROGENASE4 Integrates Cytokinin and Auxin Signaling to Control Rice Crown Root
In vivo monitoring of nitric oxide in tobacco leaves by a polyNi(II)-tetrasulfonated phthalocyanine/
Dispersive Microextraction Based on “Magnetic Water” Coupled to Gas Chromatography/Mass Spectrometry
Facile synthesis of magnetic one-dimensional polyaniline and its application in magnetic solid phase
Rapid determination of polycyclic aromatic hydrocarbons in environmental water based on magnetite na
Facile one-pot synthesis of a aptamer-based organic-silica hybrid monolithic capillary column by &qu
Quantification of 5-Methylcytosine and 5-Hydroxymethylcytosine in Genomic DNA from Hepatocellular Ca
A novel amperometric adenosine triphosphate biosensor by immobilizing graphene/dual-labeled aptamers
Isotope labelling - paired homologous double neutral loss scan-mass spectrometry for profiling of me
Electrospun polystyrene/oxidized carbon nanotubes film as both sorbent for thin film microextraction
TiO2-Based Solid Phase Extraction Strategy for Highly Effective Elimination of Normal Ribonucleoside
Determination of eight illegal drugs in human urine by combination of magnetic solid-phase extractio
Tomato SlDREB gene restricts leaf expansion and internode elongation by downregulating key genes for
Profiling of Thiol-Containing Compounds by Stable Isotope Labeling Double Precursor Ion Scan Mass Sp
Arabidopsis Plastid AMOS1/EGY1 Integrates Abscisic Acid Signaling to Regulate Global Gene Expression
Preparation of a Novel Amino-Phosphate Zwitterionic Stationary Phase for Hydrophilic Interaction Chr
Preparation of magnetic hydroxyapatite clusters and their application in the enrichment of phosphope
Determination of Endogenous Brassinosteroids in Plant Tissues Using Solid-phase Extraction with Doub
Coupling frontal elution paper chromatography with desorption corona beam ionization mass spectromet
In vivo monitoring of oxidative burst on aloe under salinity stress using hemoglobin and single-wall
Simultaneous Analysis of Multiple Endogenous Plant Hormones in Leaf Tissue of Oilseed Rape by Solid-
Highly sensitive profiling assay of acidic plant hormones using a novel mass probe by capillary elec
Quick, easy, cheap, effective, rugged and safe method with magnetic graphitized carbon black and pri
Dispersive microextraction based on magnetic polypyrrole nanowires for the fast determination of pes
Real-time monitoring of auxin vesicular exocytotic efflux from single plant protoplasts by amperomet
A highly sensitive nitric oxide biosensor based on hemoglobin-chitosan/graphene-hexadecyltrimethylam
Using pollen grains as novel hydrophilic solid-phase extraction sorbents for the simultaneous determ
“Old” metal oxide affinity chromatography as “novel” strategy for specific capture of cis-diol-conta
Determination of Oxidation Products of 5-Methylcytosine in Plants by Chemical Derivatization Coupled
Facile fabrication of reduced graphene oxide-encapsulated silica: A sorbent for solid-phase extracti
Facile Preparation of SiO2/TiO2 Composite Monolithic Capillary Column and Its Application in Enrichm
Facile preparation of organic-silica hybrid monolith for capillary hydrophilic liquid chromatography
Mutagenic and Cytotoxic Properties of Oxidation Products of 5-Methylcytosine Revealed by Next-Genera
Magnetic solid-phase extraction using magnetic hypercrosslinked polymer for rapid determination of i
Methods of sample preparation for determination of veterinary residues in food matrices by porous mo
Preparation of magnetic poly(diethyl vinylphosphonate-co-ethylene glycol dimethacrylate) for the det
Gibberellin Regulates the Arabidopsis Floral Transition through miR156-Targeted SQUAMOSA PROMOTER BI
A selective pretreatment method for determination of endogenous active brassinosteroids in plant tis
Facile synthesis of magnetic molecularly imprinted polymers and its application in magnetic solid ph
A facile approach for the polymer grafting of silica based on tandem reversible addition fragmentati
A simple sample preparation approach based on hydrophilic solid-phase extraction coupled with liquid
In-syringe dispersive solid phase extraction: a novel format for electrospun fiber based microextrac
Magnetic solid phase extraction coupled with in situ derivatization for the highly sensitive determi
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