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中文摘要：
细胞等复杂体系的直接定量成分分析是分析化学研究的热点之一。本项目工作根据研究计划，以三线性成分模型为基础，发展了新的二阶校正方法及算法，利用三维荧光光谱技术，选择不同的激发波长，用激发发射荧光光谱仪获取细胞基体等干扰因素共存下阿霉素等药物分子、氨基酸、核酸等各类荧光物质的三维荧光光谱。利用化学计量学二阶校正方法对复杂体系解析的优势，得到体系中待测各组分的相对激发光谱、相对发射荧光光谱及相对浓度曲线，在未知干扰共存下即可对感兴趣的组分进行定量预测（二阶优势），从而定量分析人工模拟细胞等体系中各感兴趣组成成分，并对其动态过程进行近实时定性定量解析，实现分析研究方法的突破，有效地解决细胞等研究实际中遇到的定量分析难题。开发的绿色定量分析方法具有较强的原始创新性。同时对本实验室的相关工作做了系统的总结，对不同的算法进行了比较。与阿根廷学者合作，由我们提供三种核心数学分离组件，共同发布了一个MATLAB图形界面工具包，用于二阶校正的推广应用，通过考察各种三线性成分分解及其二阶校正方法及理论在生命科学、环境科学、医学、食品科学等领域的创新性应用前景，认为十分广阔。
结论摘要：
英文主题词Three-dimensional fluorescence; cell matrix; Dynamic resolution; Component measurement；Second-order calibrarion
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期刊论文
Simultaneous determination of 6-methylcoumarin and 7-methoxycoumarin in cosmetics using three-dimens
Using near-infrared spectroscopy and differential adsorption bed method to study adsorption kinetics
Quantitative analysis of noradrenaline in human plasma samples using kinetic fluorometric methods co
Rapid determination of thiabendazole in orange extract using excitation-emission matrix fluorescence
Highly sensitive fluorescence quantification of irinotecan in biological fluids with the aid of seco
Self-weighted alternating normalized residue fitting algorithm with application to quantitative anal
The chemical rank estimation for excitation-emission matrix fluorescence data by region-based moving
Rapid Determination of Bitertanol Content in Banana Using Three-Dimensional Fluorescence Coupled wit
Fluorescent quantification of terazosin hydrochloride content in human plasma and tablets using seco
Determination of Indole-3-Acetic Acid in Soil Using Excitation-Emission Matrix Fluorescence with Tri
Interference-free determination of abscisic acid and gibberellin in plant samples using excitation-e
Quantitative Analysis of Epinephrine in Human Plasma Samples Using Kinetic Fluorometric Method Combi
Studying the Interaction of Pirarubicin with DNA and Determining Pirarubicin in Human Urine Samples:
Simultaneous determination of psoralen and isopsoralen in plasma and Chinese medicine Man Ling Gu Ba
Quantitative analysis of levodopa, carbidopa and methyldopa in human plasma samples using HPLC-DAD c
Quantitative analysis of triazine herbicides in environmental samples by using high performance liqu
Automatic configuration of optimized sample-weighted least-squares support vector machine by particl
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