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中文摘要：
我们运用real-time RT-PCR 方法考察了可能靶向作用于肝素酶基因的内源性miRNA分子，并在大样本胃癌组织中筛查出差异表达的miR-148a，miR-148b，miR-152和miR-203,运用统计学方法分析后，我们发现以上4个miRNA分子与胃癌的癌灶大小密切相关；此外，miR-152和miR-203还与胃癌的浸润深度密切相关。我们进一步在体外胃癌细胞系中过表达miR-148b,结果表明miR-148b能显著抑制胃癌细胞的增殖，这在裸鼠成瘤实验中也得到了证实。在此基础上，我们运用生物信息学分析miR-148b可能调控的靶基因，并构建荧光素酶载体与miR-148b进行共转染，初步推断胆囊收缩素-B/胃泌素受体(CCKBR)可能受miR-148b调控。此外，我们用western blot也验证了miR-148b在转录后水平负性调控CCKBR表达。我们筛选出一组与胃癌发生、转移密切相关的候选microRNAs，确定它们的作用机制，可用于胃癌高危个体监测、早诊和转移预测的基因标志，为进一步制订胃癌合理高效的综合治疗方案提供了新的线索。
结论摘要：
英文主题词microRNA;gastric cancer;heparanase
成果综合统计
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期刊论文
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