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中文摘要：
针对目前免疫分析现场即时检测（POCT）所面临的成本与准确性、便携性不协调的瓶颈问题，本项目在微流控纸芯片平台上引入电化学发光分析方法，制备开发低成本、高灵敏度、方便快捷的微流控纸芯片电化学发光POCT器件，建立免疫电化学发光分析的微流控纸芯片实验室。设计制备具有高性能的纸上三电极和亲水微通道的微流控纸芯片，在纸芯片上组装免疫分析抗体，构建一维电化学发光微流控纸芯片器件；研究抗体的纸上电化学发光标记原理和方法，发展兼有分子识别和电化学发光信号的抗体，利用抗原抗体的特异识别和电压的控制解决分析方法的选择性,据此研发高选择性的免疫电化学发光微流控纸芯片实验室；揭示纸上电化学发光免疫检测机理，进一步发展三维免疫电化学发光微流控纸芯片实验室，探索对多指标的疾病诊断与复杂诊断过程的现场即时分析和多组分的同时检测，研究纸芯片与外部便携式设备的偶联，为实现纸上免疫分析POCT技术奠定理论与技术基础。
英文摘要：
microfluidic paper chip；electrochemiluminescence；immnoassay；electrochemical analysis；colorimetric
结论摘要：
在本项目中，优化了微流控纸芯片中亲水微通道的形状、位置、分布等参数；设计制备了微流控纸芯片；考察了纸上丝网印刷三电极的边缘形貌特征以及电化学性能；开拓集分离、富集、洗涤、检测多功能于一体的多功能、高通量、可寻址阵列 3D 纸芯片的制备；设计制备了三维中空通道微流控纸芯片。在设计制备的微流控纸芯片中，构建了基于微流控纸芯片三电极体系的电化学发光免疫分析方法；通过设计、制备、优化的微流控免疫电化学发光纸芯片电化学发光性能的研究，提出了相应的纸上免疫电化学发光检测机理。同时，构建了多种化学发光、电化学微流控免疫纸芯片传感器，实现了纸芯片上多种分析方法的定量分析；围绕纳米材料的制备、功能组装与修饰进行了研究，并对这些纳米材料的性能及在纸芯片上应用进行了研究。合成了多种贵金属纳米材料，碳纳米材料和半导体纳米材料，并通过多种途径对制备的纳米材料进行修饰功能化；依据功能化后的纳米材料的物理性质、化学性质以及光电性质将这些纳米材料进行应用，构建不同类型的传感器；对实验体系进行修改优化；结合吸收光谱、电化学、电致化学发光、光致电等分析方法，在纸芯片上构建了一系列高灵敏和好的稳定性的免疫传感器。
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期刊论文
Three-dimensional paper-based electrochemiluminescence immunodevice for multiplexed measurement of b
Ultrasensitive Electrochemical Immunosensor based on Au nanoparticals dotted carbon nanotube-graphen
Paper-Based Electrochemiluminescent 3D Immunodevice for Lab-on-Paper, Specific, and Sensitive Point-
Paper-based chemiluminescence ELISA: Lab-on-paper based on chitosan modi？ed paper device and wax-scr
Electrogenerated Chemiluminescence from phenyleneethynylene derivative and Its Ultrasensitive Immuno
Magnetic beads-based electrochemiluminescence immunosensor for determination of cancer markers using
A sensitive signal-off aptasensor for adenosine triphosphate based on the quenching of Ru(bpy)32+-do
Ultrasensitive Electrochemiluminescence Immunoassay for Protein Specific Detection Based on Dendrime
“Signal-off” photoelectrochemical DNA sensing strategy based on target dependent DNA probe conformat
Self-powered competitive immunosensor driven by biofuel cell based on hollow-channel paper analytica
Highly sensitive chemiluminescence immunoassay on chitosan membrane modified paper platform using Ti
Molecularly Imprinted Polymer Grafted Porous Au-Paper Electrode for an Microfluidic Electro-Analytic
A disposable simultaneous electrochemical sensor array based on a molecularly imprinted film at a NH
Visible light photoelectrochemical sensor based on Au nanoparticles and molecularly imprinted poly(o
A chemiluminescence excited photoelectrochemistry aptamer-device equipped with a tin dioxide quantum
Ultrasensitive electrochemiluminescent immunosensor based on dual signal amplification strategy of g
Chemiluminescence excited photoelectrochemical competitive immunosensing lab-on-paper device using a
Aptamer-Based electrochemiluminescent detection of MCF-7 cancer cells based on carbon quantum dots c
Simple and covalent fabrication of a paper device and its application in sensitive chemiluminescence
Ultrasensitive electrochemiluminescence immunosensor based on nanoporous gold electrode and Ru-AuNPs
3D origami electrochemiluminescence immunodevice based on porous silver-paper electrode and nanoporo
A novel microfluidic origami photoelectrochemical sensor based on CdTe quantum dots modified molecul
An origami electrochemiluminescence immunosensor based on gold/graphene for specific, sensitive poin
Electrochemiluminescence of peroxydisulfate using flower-like Ag@Au-paper electrode and Pd@Au-assist
Au-Pt nanoparticle-based electrochemiluminescence immunoassay of a cancer biomarker using ZnO nanosp
Electrochemical device based on a Pt nanosphere-paper working electrode for in situ and real-time de
Highly sensitive hybridization assay using the electrochemiluminescence of an ITO electrode, CdTe qu
Magnetic nanoparticle-based electrochemiluminescent immunosensor for detection of carcinoembryonic a
Application of indium tin oxide device in gold-coated magnetic iron solid support enhanced electroch
Microfluidic paper-based multiplex colorimetric immunodevice based on the catalytic effect of Pd/Fe3
Application of ZnO quantum dots dotted carbon nanotube for sensitive electrochemiluminescence immuno
Photoelectrochemical sensor for pentachlorophenol on microfluidic paper-based analytical device base
Core–shell Fe3O4–Au magnetic nanoparticles based nonenzymatic ultrasensitive electrochemiluminescenc
Multiplexed sandwich immunoassays using flow-injection electrochemiluminescence with designed substr
3D microfluidic origami electrochemiluminescence immunodevice for sensitive point-of-care testing of
3D origami electrochemical immunodevice for sensitive point-of-care testing based on dual-signal amp
Paper-based electrochemiluminescence origami cyto-device for multiple cancer cells detection using p
Paper-based electrochemical immunosensor for carcinoembryonic antigen based on three dimensional flo
Graphene functionalized porous Au-paper based electrochemiluminescence device for detection of DNA u
A photoelectrochemical biosensor using ruthenium complex-reduced graphene oxide hybrid as the photoc
Multiplex electrochemical origami immunodevice based on cuboid silver-paper electrode and metal ions
Gold-silver nanocomposite-functionalized graphene sensing platform for an electrochemiluminescent im
Ultrasensitive electrochemiluminescence immunoassay for tumor marker based on quantum dots coated ca
Electrophoretic separation in a microfluidic paper-based analytical device with an on-column wireles
Paper-based electrochemiluminescence immunodevice for carcinoembryonic antigen using nanoporous gold
Electropolymerized Poly(3,4-ethylendioxythiophene)/Graphene Composite Film and its Application in Qu
A three-dimensional origami-based immuno-biofuel cell for self-powered, low-cost, and sensitive poin
Facile and sensitive paper-based chemiluminescence DNA biosensor using carbon dots dotted nanoporous
Disposable electrochemical immunosensor for simultaneous assay of a panel of breast cancer tumor mar
Multiplexed enzyme-free electrochemical immunosensor based on ZnO nanorods modified reduced graphene
Application of nanoporous Pd as catalytically promoted nanolabels for ultrasensitive electrochemilum
Electrochemiluminescence device for in-situ and accurate determination of CA153 at the MCF-7 cell su
Chemiluminescence excited paper-based photoelectrochemical competitive immunosensing based on porous
Flexible paper-based ZnO nanorod light-emitting diodes induced multiplexed photoelectrochemical immu
Photoelectrochemical Lab-on-Paper Device Based on an Integrated Paper Supercapacitor and Internal Li
Photoelectrochemical lab-on-paper device equipped with a porous Au-paper electrode and fluidic delay
Photoelectrochemical Sensor Based on Molecularly Imprinted Polymer-Coated TiO2 Nanotubes for Lindane
A near-infrared light photoelectrochemical immunosensor based on a Au-paper electrode and naphthaloc
Rechargeable battery-triggered electrochemiluminescence detection on microfluidic origami immunodevi
Paper-based photoelectrochemical immunosensing based on CdS QD sensitized multidimensional porous Zn
Ultrasensitive detection of lead ion sensor based on gold nanodendrites modified electrode and elect
CuO-induced signal amplification strategy for multiplexed photoelectrochemical immunosensing using C
Electrochemical DNA sensor based on three-dimensional folding paper device for specific and sensitiv
Paper-based three-dimensional electrochemical immunodevice based on multi-walled carbon nanotubes fu
Electrochemiluminescence of blue-luminescent graphene quantum dots and its application in ultrasensi
Synthesis and characterization of graphene nanosheets attached to spiky MnO2 nanospheres and its app
A disposable immunosensor device for point-of-care test of tumor marker based on copper-mediated amp
In situ assembly of porous Au-paper electrode and functionalization of magnetic silica nanoparticles
Electrochemical immunosensor based on graphene–polyaniline composites and carboxylated graphene oxid
“Sugarcoated haws on a stick”-like MWNTs–Fe3O4–C coaxial nanomaterial: Synthesis, characterization a
A 3D origami electrochemical immunodevice based on a Au@Pd alloy nanoparticle-paper electrode for th
A visible light photoelectrochemical sensor for tumor marker detection using tin dioxide quantum dot
A 3D origami multiple electrochemiluminescence immunodevice based on a porous silver-paper electrode
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