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中文摘要：
脐带血干/祖细胞具有重要的临床应用价值，但其含量非常有限，急需有效扩增。本项目围绕造血干/祖细胞体外扩增的适宜条件进行了多项研究。首先采用transwell和微胶囊包埋基质细胞两种技术与造血干/祖细胞进行间接共培养，证实基质细胞与造血干细胞共培养的有效作用距离在350微米左右，并确定了微胶囊化共培养体系的适宜细胞接种密度、微胶珠的适宜尺度等关键参数。微胶囊化基质细胞与造血干/祖细胞在旋转壁式生物反应器内共培养是最佳的扩增培养系统。进而为了充分利用脐带血资源，还对同时收获培养脐带血中两种重要干细胞开展了研究，优化了脐带血间充质干细胞体外分离培养条件，并实现了生物反应器内同时培养与收获脐带血来源间充质干细胞及造血干细胞。最后，为了进一步在体外模拟骨髓造血微环境从而有效扩增造血干/祖细胞，本项目对成骨细胞支持造血干/祖细胞扩增进行了系统的研究，明确了成骨细胞分泌各种细胞因子以支持造血的扩增机理。本研究在采用微胶囊技术与造血干细胞共培养、使用旋转壁式生物反应器对造血干/祖细胞进行共培养及扩增、微胶囊化成骨细胞模拟骨髓造血微环境等方面处于国际领先水平，为体外有效扩增造血干/祖细胞开辟了新方法。
结论摘要：
英文主题词hematopoietic stem cells; bioreactor; expansion; cord blood; microcapsules
成果综合统计
期刊论文
会议论文
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期刊论文
Effect of protocatechuic acid from Alpinia oxyphylla on proliferation of human adipose tissue-derive
Effective expansion of umbilical cord blood hematopoietic stem/progenitor cells by regulation of mic
Optimization of Primary Culture Condition for Mesenchymal Stem Cells Derived from Umbilical Cord Blo
Effects of encapsulated rabbit mesenchymal stem cells on ex vivo expansion of human umbilical cord b
Preparation, fabrication and biocompatibility of novel injectable temperature-sensitive chitosan/gly
Enhancement of Adipose-Derived Stem Cell Differentiation in Scaffolds with IGF-I Gene Impregnation U
Microencapsulated Osteoblasts Support Hematopoietic Stem/Progenitor Cell Expansion in Hypoxic Enviro
Simultaneous expansion and harvest of hematopoietic stem cells and mesenchymal stem cells derived fr
Collagen-chitosan polymer as a scaffold for the proliferation of human adipose tissue-derived stem c
Differentiation Enhancement of ADSC in Scaffolds With IGF-1 Gene Impregnation Under Dynamic Microenv
Investigation of the effective action distance between hematopoietic stem/progenitor cells and human
Optimization for dissociation and culture of mesenchymal stem cells derived from umbilical cord Bloo
会议论文
Effects of encapsulated rabbit mesenchymal stem cells on ex vivo expansion of human umbilical cord b
Ex vivo expansion of human umbilical cord blood hematopoietic stem/progenitor with support of microe
Effect of surface modification on morphology, mechanism and function of human adipose-derived stem c
Preparation, detection and controlled release of PLGA microspheres-based scaffolds embedded with BSA
Numerical simulation and analysis of fluid field in a rotating bioreactor on three-dimensional fabri
Effective expansion of umbilical cord blood HSPCs by regulating of microencapsulated osteoblasts und
Ex vivo expansion of umbilical cord blood HSCs by supporting of osteblasts under low oxygen conditio
Hypoxic culture and expansion of mesenchymal stem cells in airlift loop hollow fiber membrane biorea
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