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中文摘要：
为了提高和推广中药治疗肿瘤疾病的水平，需要深入了解中药抑制肿瘤生长和转移的分子机理。本项目拟采用质粒转染技术和多种荧光技术在单个活细胞中实时研究中药诱导肿瘤细胞凋亡的早期分子调控机理。拟选择肺腺癌细胞株作为研究模型，中药选择消癌平。将基于GFPs构建的Bax、Bid和caspases等靶基因质粒转染细胞，然后利用荧光共聚焦扫描显微镜、荧光共振能量转移（FRET）、荧光相关谱（FCS）、全内反射荧光
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期刊论文
TNFα Induces Apoptosis Through JNK/Bax-Dependent Pathway in Differentiated, but Not Na?ve PC12 Cells
Rapid determination of seed vigor based on the level of superoxide generation
during early imbibitio
Quantitative Analysis of Fluorescence Resonance Energy Transfer (FRET) Efficiency by Fitting Fluores
Bufalin Induces Reactive Oxygen Species (ROS)-Dependent Bax Transloc- ation and apoptosis in ASTC-a-
FLIM and emission spectral analysis of caspase-3 activation inside single living cell during antican
Live Morphological Analysis of Taxol-Induced Cytoplasmic Vacuoliaz- ation in human lung adenocarcino
Fluorescence Emission Analysis of Caspase-3 Activation Induced by Xiao-Ai-Ping (XAP) Inside Living H
Taxol induces caspase-independe- nt cytoplasmic vacuolization and cell death through endoplasmic ret
Spatio-temporal dynamic analysis of Bid activation and apoptosis induced by alkaline condition in hu
会议论文
Fluorescence Imaging Analysis of Taxol-Induced Cell Death with Cell Swelling and Cytoplasmic Vacuoli
Analysis of caspase3 activation in ChanSu-induced Apoptosis of ASTC-a-1 Cells by Fluorescence Techni
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