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中文摘要：
肠致病性大肠杆菌（EPEC）黏附于肠黏膜表面是其进一步定植引起婴幼儿腹泻和成人腹泻的的前提条件。近年来研究发现母乳低聚糖（HMO）和一些人工合成的低聚糖可以抑制EPEC对肠道的黏附，但对其抗黏附作用的构效关系和内在机制了解甚微。本研究拟以课题组自主分离鉴定的瑞士乳杆菌所产胞外多糖为研究对象，利用内切聚糖酶对其进行可控酶水解，通过优化酶解条件，控制多糖的水解度，暴露活性低聚糖片段，制备结构上与HMO相似的低聚糖；在对酶解产物进行分离纯化及结构鉴定的基础上，通过体外EPEC竞争性黏附细胞实验以及体内EPEC侵染小鼠等实验，定性、定位、定量分析EPEC肠细胞脱落位点毒力岛基因群中与黏附相关基因和蛋白的表达以及小鼠肠道黏膜免疫球蛋白和特征细胞因子的变化，获得低聚糖抗EPEC黏附作用的构效关系，并揭示二者间的内在机制。本研究对于婴幼儿配方奶粉母乳化以及乳酸菌多糖的深层次开发具有重要的理论和实际意义。
结论摘要：
英文主题词Lactobacillus helveticus MB2-1；Exopolysaccharide；Structural characterization；Enteropathogenic Escherichia coli；Anti-adhesive activity
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期刊论文
Lactobacillus plantarum 70810 from Chinese paocai as a potential source of beta-galactosidase for pr
Characterization of a novel polysaccharide with anti-colon cancer activity from Lactobacillus helvet
Production of exopolysaccharides by Lactobacillus helveticus MB2-1 and its functional characteristic
Structural characterization and bioactivity of released exopolysaccharides from Lactobacillus planta
Structural elucidation and antioxidant activities of exopolysaccharides from Lactobacillus helveticu
Characterization of a novel exopolysaccharide with antitumor activity from Lactobacillus plantarum 7
Chemical modification, characterization and bioactivity of a released exopolysaccharide (r-EPS1) fro
Influences of structures of galactooligosaccharides and fructooligosaccharides on the fermentation i
Lactosucrose and its analogues derived from lactose and sucrose: Influence of structure on human int
Complete genome sequence of Lactobacillus helveticus MB2-1, a probiotic bacterium producing exopolys
Structural characterization and anticancer activity of cell-bound exopolysaccharide from Lactobacill
Characterization of an antiproliferative exopolysaccharide (LHEPS-2) from Lactobacillus helveticus M
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