欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
位置：立项数据库 > 立项详情页
中文摘要：
龋齿病是累及全人类的细菌感染性疾病，WHO已将其列入危害人类健康的三大疾病仅次于心脑血管病和癌症，我国卫生部和国家教委已把龋齿列为2000年学生重点防治疾病之一。大量研究证明变形链球菌是最主要的致龋细菌，对其进行大规模蛋白结构研究将深化对变链菌致龋分子机理的理解，有助于寻找龋病防治的有效靶标以及可作疫苗的抗原蛋白。经多年努力建成的北京大学结构基因组学平台可进行每年1000个以上基因克隆及表达，能够胜任变链菌的全基因组蛋白结构研究，并在2004年进行了200多个变链菌基因的先导研究。在此基础上，拟进行1）变链菌全基因组非膜蛋白基因约1500个的大规模质粒构建；2）高通量表达、纯化可溶性蛋白；3）培养蛋白晶体，对衍射好的晶体进行结构解析；4）在结构基础上，对未知功能的蛋白进行深入分析，由结构信息推动功能研究
结论摘要：
英文主题词S. mutans; structural genomics;structural genomics platform; cloning,expression and protein purification; crystallography
成果综合统计
期刊论文
会议论文
专利
获奖
著作
期刊论文
Crystallization and preliminary x-ray crystallographic studies of SMU.134 protein from caries pathog
Crystallization and preliminary crystallographic analysis of D-alanine-D-alanine ligase from Strepto
Protein preparation and preliminary X-ray crystallographic analysis of a putative glucosamine 6-phos
Protein preparation, crystallization and preliminary X-ray crystallographic analysis of SMU.961 prot
Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu_195c fro
Preparation, crystallization and preliminary X-ray analysis of threonine synthase from Streptococcus
Protein preparation, crystallization and preliminary X-ray analysis of the C-terminal domain of huma
Structure of a fatty-acid-binding protein from Bacillus subtilis determined by sulfur-SAD phasing us
Preliminary X-ray crystallographic analysis of a nitric oxide-inducible lactate dehydrogenase from S
Crystal Structures of Phosphotransferase System Enzymes PtxB (IIB(Asc)) and PtxA (IIA(Asc)) from Str
Preliminary crystallographic studies of purine nucleoside phosphorylase from the cariogenic pathogen
Crystal structures of Streptococcus mutans 2 '-deoxycytidylate deaminase and its complex with substr
Preliminary X-ray crystallographic analysis of SMU.573, a putative sugar kinase from Streptococcus m
Protein preparation, crystallization and preliminary X-ray crystallographic analysis of N-acetylglut
Preparation, crystallization and preliminary X-ray analysis of the methionine synthase (MetE) from S
Open-closed conformational change revealed by the crystal structures of 3-keto-L-gulonate 6-phosphat
相关项目
苏晓东的项目