中文摘要：

目的：应用高效液相-二极管阵列法（HPLC-DAD）建立同时测定酸枣仁合欢方（SHF）抗抑郁皂苷部位中氧化芍药苷、芍药内酯苷、芍药苷、（-）-丁香树脂酚-4-O-β-D-呋喃芹糖基-（1→2）-β-D-吡喃葡萄糖苷、白桦酯酸等5个指标成分含量的分析方法,并指认5个指标成分的来源,以建立SHF抗抑郁有效部位的质量控制方法。方法：色谱柱：Agilent TC-C18柱（规格：4.6 mm×250 mm,5μm）;流动相：乙腈（A）-0.1%磷酸水;梯度洗脱,运行时间：85 min,洗脱程序为：0~9 min,7%~14%A;9~20min,14%A;20~31 min,14%~20%A;31~32 min,20%~23%A;32~44 min,23%~32%A;44~52 min,32%~36%A;52~65 min,36%~63%A;65~85 min,63%~100%A。流速：1 m L·min-1;检测波长：204 nm;柱温：30℃;进样量：20μL。通过比较各单味药材皂苷部位与缺单味药的阴性皂苷部位、混合标准品、全方皂苷部位的HPLC图,指认出5个指标成分的来源。结果：在上述色谱条件下各成分峰均能达到分离,分离度良好。在各自的测定浓度范围内,SHF抗抑郁皂苷部位中5个指标成分均呈现良好的线性关系（R2〉0.999）,其回收率均不小于97.0%。5个指标成分中氧化芍药苷、芍药内酯苷、芍药苷均来源于白芍;（-）-丁香树脂酚-4-O-β-D-呋喃芹糖基-（1→2）-β-D-吡喃葡萄糖苷全部来源于合欢皮;白桦酯酸在4味药材中均有体现。结论：HPLC-DAD测定方法快速、灵敏、重现性良好,实现了一次进样同时测定SHF抗抑郁皂苷部位中5种指标成分含量,为研制简单有效、质量可控的创新中药奠定了基础。

英文摘要：

Objective： To establish an HPLC method for the simultaneous determination of oxypaeoniflora, albiflorin, paeoniflorin, （-）-syringaresnol-4-O-β-D-apiofuranosyl-（1→2）-β-D-glucopyranoside and betulinic acid in suanzaorenhehuanfang （SHF） Saponin with HPLC-DAD analysis and identify the sources of the 5 constituents. Furthermore, the quality control standard of SHF Saponin was established, which has anti-depression effect. Methods： HPLC-DAD analysis was performed on an Agilent TC-C18 column （4.6 mm × 250 mm, 5 μm）. The mobile phase： acetonitrile （A） -0.1% phosphoric acid aqueous solution. The elution mode was gradient elution： 0～9 min, 7%～14% A; 9～20 min, 14% A; 20～31 min, 14%～20% A; 31～32 min, 20%～23% A; 32～44 min, 23%～32% A; 44～52 min, 32% A～36%; 52～65 min, 36%～63% A; 65～85 min, 63%～100% A. The flow rate： 1 mL×min-1. The detection wavelength： 204 nm. The column temperature： 30 ℃. The injection volume： 20 μL. The sources of the 5 constituents were identified by comparing the HPLC chromatogram of the saponin of a single herb, the negative saponin lack of 1 herb, mixture reference substance and SHF Saponin. Results： In this condition, each peak was well separated and the 5 constituents had good linear relation with their determination of the concentration （R2〉0.999）. The recovery rate was not less than 97.0%. Among 5 constituents, oxypaeoniflora, albiflorin and paeoniflorin belonged to Radix Paeoniae Alba （dried root of Ranunculaceae peony Paeonia lactiflora Pall）. （-）-Syringaresnol-4-O-β-D-apiofuranosyl-（1→2） -β- D-glucopyranoside belonged to Cortex Albiziae （dried stem bark of the leguminous plant Albizzia julibrissin Durazz）. Besides, betulinic acid belonged to 4 herbs of SHF. Conclusion： The method is fast, sensitive and reproducible, could make simultaneous determination of 6 constituents of SHF Saponin possible. It also provides a theoretical basis and experimental basis for the development of effective, quali