中文摘要：

以卵白蛋白为原料,采用胃蛋白酶和胰蛋白酶复合水解,对其水解产物进行体外抑菌试验,并利用AKTA蛋白纯化系统、透析及RP-HPLC分离得到2个具有抗菌活性的组分F21和F22.考察了2种组分对大肠杆菌细胞膜渗透性和细胞膜完整性的影响,及其DPPH·自由基清除能力.50 μg/mL的F21和F22组分对大肠杆菌分别具有84.6％和22.5％的抑菌率,对金黄色葡萄球菌分别具有53.3％和29.6％的抑菌率.半乳糖苷酶释放法和流式细胞仪分析结果表明,2种产物均能破坏大肠杆菌细胞膜渗透性和细胞膜完整性.此外,F21和F22组分均具有抗氧化能力,能有效清除DPPH·自由基,当质量浓度为2.0 mg/mL时,两者DPPH·清除率接近于1.0mg/mL GSH的DPPH·清除效果.卵白蛋白水解产物可作为抗菌剂和抗氧化剂加以深度开发利用.

英文摘要：

Ovalbumin hydrolyzate was prepared by pepsin and trypsin hydrolysis.Two hydrolyzate fractions with antibacterial activity F21 and F22,were separated by A KTA protein purification systems,dialysis,and RP-HPLC.The effects of F21 and F22 on cell membrane permeability and membrane integrity and their DPPH o radical scavenging ability were also investigated.The inhibition rates of 50 μg/mL of F21 and F22 on Escherichia coli were 84.6% and 22.5% respectively,and the inhibition rates on Staphylococcus aureus were 53.3% and 29.6%.Galactosidase release and flow cytometry analysis showed that the two fractions could destroy Escherichia coli cell membrane permeability and membrane integrity.In addition,they could effectively scavenge DPPH o radical.When the concentrations were 2.0 mg/mL,the DPPH o radical scavenging abilities of the two fractions were close to the scavenging ability of 1.0 mg/mL GSH.As a result,ovalbumin hydrolysate could be used as antibacterial agents and antioxidants in further exploitation.