中文摘要：

以κ-卡拉胶为原料,经盐酸水解得到一系列寡糖混合物.以3-氨基-9-乙基咔唑（3-amino-9-ethylcarbazole,AEC）为衍生化试剂,对酸解得到的κ-卡拉胶寡糖进行柱前衍生,采用反相C18色谱柱（250 mm×4.6 mm,5μm）,乙腈和乙酸铵水溶液（pH 4.5）为流动相,梯度洗脱,在254 nm波长处检测,建立了κ-卡拉胶寡糖衍生物的高效液相色谱（HPLC）分离以及液相色谱-电喷雾质谱联用（LC-ESI-MS）分离分析的方法,并对AEC衍生后的κ-卡拉胶寡糖进行多级质谱裂解（MSn）,进一步获得了κ-卡拉胶寡糖的结构信息.该方法对κ-卡拉胶寡糖的结构分析、构效关系等方面的研究有参考价值.

英文摘要：

A series of κ-carrageenan oligosaccharides were prepared by using hydrochloric acid hydrolysis.The κ-carrageenan oligosaccharides derivatized with 3-amino-9-ethylcarbazole（AEC） were analyzed by high performance liquid chromatography（HPLC） and HPLC coupling with electrospray ionization ion-trap mass spectrometry（LC-ESI-MS）.The HPLC separation was carried out on a reverse phase（RP） C18 column（250 mm×4.6 mm,5 μm） in a linear gradient mode,using acetonitrile and ammonium acetate aqueous solu-tion（pH 4.5） as mobile phase and a ultraviolet（UV） detection at 254 nm.Furthermore,the structure of the κ-carrageenan oligosaccharide was confirmed by multi-stage mass spectrometry（MSn）.These results may be helpful to investigation in structure identification and structure-activity relationship of κ-carrageenan oligo-saccharides.