中文摘要：

目的 研究肝豆灵对Wilson病（Wilson＇s disease,WD）大鼠肝组织miRNA-122表达的影响,探究其保护肝脏的机制.方法 将24只Wistar大鼠随机分为空白对照组、模型组、青霉胺组和肝豆灵组.铜负荷喂养8周后,分别用青霉胺、肝豆灵以成人等效剂量灌胃,空白对照组及模型组均以等量生理盐水灌胃,每组均灌胃4周.观察各组大鼠肝功能及肝组织miRNA-122的表达水平.结果 肝豆灵和青霉胺均可显著降低WD大鼠异常升高的血清丙氨酸氨基转移酶（alanine aminotransferase,ALT）、天冬氨酸氨基转移酶（aspartate aminotransferase,AST）、总胆红素（total bilirubin,TBil）（P＜0.05,或P＜0.01）,显著升高WD大鼠肝组织异常降低的miRNA-122表达水平（P＜0.01）.肝豆灵降低血清ALT、AST和TBil的作用及升高肝组织miRNA 122表达的作用显著优于青霉胺（P＜0.05,或P＜0.01）.结论 肝豆灵保护WD大鼠肝功能的作用与其上调肝组织miRNA-122的表达有关.

英文摘要：

Objective To study the effect of Gandouling on the miRNA-122 expression in liver tissues of rats with Wilson＇s disease （WD） and to explore the action mechanism of Gandouling in liver protection.Methods Twenty-four Wistar rats were randomly divided into blank control group,model group,penicillamine group,and Gandouling group.All rats except those in the blank control group were given copper-overloaded diets for 8 weeks.Then,the penicillamine group and Gandouling group received penicillamine and Gandouling,respectively,by gavage at human-equivalent doses,while the blank control group and model group received an equal volume of normal saline by gavage; intragastric administration was performed for 4 weeks in each group.The liver function and hepatic miRNA-122 expression in each group were measured.Results Both Gandouling and penicillamine significantly reduced the levels of alanine aminotransferase （ALT）,aspartate aminotransferase （AST）,and total bilirubin （TBil） （P＜0.05 or P＜0.01）,which were abnormally elevated in rats with WD,and significantly increased miRNA-122 expression （P＜0.01）,which was significantly reduced in the liver tissues of rats with WD.Gandouling was significantly superior to penicillamine in reducing serum ALT,AST,and TBil levels and increasing hepatic miRNA-122 expression （P＜0.05 or P＜0.01）.Conclusion Gandouling can protect the liver function of rats with WD,possibly by increasing hepatic miRNA-122 expression.