中文摘要：

目的利用代谢组学技术研究铜负荷大鼠肝组织的小分子变化,探讨铜过量对肝脏小分子代谢的影响。方法 16只Wistar大鼠随机分为正常组和模型组,以铜负荷法造模,通过核磁共振氢谱技术采集大鼠肝组织的代谢轮廓,以PLS-DA方法分析铜中毒后,大鼠肝组织代谢物的变化。结果与正常组对比,模型组大鼠肝组织中尿素囊、牛磺酸、肌醇、赖氨酸、尼克酰胺、乙醇胺、乙酸、谷氨酸、酪氨酸、尿苷、甲硫氨酸、苏氨酸、异亮氨酸、3-羟基丁酸、缬氨酸、乳酸、亮氨酸、苯丙氨酸、N-乙酰天冬氨酸、延胡索酸和腺苷/肌苷的含量升高（P〈0.05）,肌酸、天冬酰胺、天冬氨酸、磷酸胆碱和甘露醇的含量降低（P〈0.05）。结论铜对大鼠肝组织的损伤可能涉及鸟氨酸和三羧酸循环、磷脂、氨基酸、能量、核苷酸和糖的代谢。

英文摘要：

Objective To explore the changes of small molecules in liver tissues of rats with copper overload and the influences of copper overload on metabolism of small molecules in liver tissues. Methods Sixteen Wistar rats were randomly divided into normal control group and model group. Copper overload method was used to establish the model rats.1H-NMR was used to acquire the metabolic profile of rat liver tissues,and PLS-DA was used to analyze the changes of metabolites in rat liver tissues after copper poisoning. Results Compared with normal control group,the contents of allantoin,taurine,myoinositol,lysine,nicotinamide,ethanolamine,acetate,glutamate,tyrosine,uridine,methionine,threonine,isoleucine,3-hydroxybutyrate,valine,lactate,leucine,phenylalanine,Nacetylaspartate,fumarate,and adenosine / inosine were increased（ P〈0. 05）,while the contents of creatine,asparagine,aspartate,phosphorylcholine and mannitol were decreased in model group（ P〈0. 05）. Conclusion Copper damage in rat liver tissues may be involved in the metabolism of ornithine and Krebs cycles,lecithin,amino acid,energy,nucleotides and glucose.