中文摘要：

目的 观察苦参碱（Ma）联用顺铂（DDP）对人肝癌裸鼠移植瘤生长的影响并探讨其作用的可能分子机制. 方法 将人肝癌细胞株HepG2注入BALB/c裸鼠腋部皮下建立移植瘤模型,待成瘤后,随机分为4组：对照组、Ma组、DDP组及Ma和DDP联合组,均采用腹腔注射给药.期间观察记录裸鼠的体质量和肿瘤体积,绘制体质量变化曲线与瘤体生长曲线图;用药14d后处死实验裸鼠称瘤重,计算抑瘤率;免疫组织化学法检测瘤组织中凋亡相关蛋白存活素与半胱氨酸-天冬氨酸蛋白酶-3 （caspase-3）的表达.多组间数据比较采用单因素方差（ANOVA）分析,两组间比较采用两独立样本t检验. 结果 Ma或DDP单用对裸鼠移植瘤抑瘤率分别为37.5％和75.0％,Ma和DDP联用可明显抑制瘤体生长（P＜0.05）,其抑瘤率达83.3％,明显高于Ma或DDP单用组.Ma＋DDP组裸鼠的平均体质量为21.50 g,比对照组（28.50 g）及Ma组（26.67 g）低,但较DDP组（17.33 g）高,且精神、活动、食量等全身状况也明显优于DDP组,瘤组织中细胞存活素阳性率为19.58％±4.52％,较对照组（83.26％±15.56％）、Ma组（62.50％±8.09％）及DDP组（38.67％±8.26％）降低（P＜0.05）.与之相对,NS组、Ma组及DDP组caspase-3的阳性细胞率分别为21.15％±3.68％、35.13％±10.57％、65.88％±4.81％,而Ma＋DDP组较之明显升高,为78.26％±6.09％,Ma＋DDP组与其他组比较,差异均具有统计学意义（P＜0.05）. 结论 Ma或DDP单用对裸鼠移植瘤具有抑制作用,Ma与DDP联用能增强DDP对裸鼠移植瘤的抑瘤作用和改善全身状况,降低DDP的不良反应.其可能的作用机制是影响存活素和caspase-3的表达,诱导肿瘤细胞凋亡.

英文摘要：

Objective To investigate the effect and molecular mechanism of cisplatin （DDP） combined with Matrine （Ma;plant alkaloid） against hepatocellular carcinoma using a nude mouse model with xenografted human tumors.Methods Twenty-four 6-week old male BALB/c nude mice were subcutaneously injected with HepG2 cells into the axilla,and randomly divided into four groups：control （NS） group,Ma treatment group,DDP treatment group and DDP＋Ma combination treatment group.All treatments were delivered via intraperitoneal injection.Changes in whole body weights and tumor volume were assessed by before and after treatment measurements and plotting of growth curves.After 14 days of drug intervention,the mice were sacrificed for collection of tumor tissue and assessment of the tumor inhibition rates for each treatment.Affects on expression of survivin and caspase-3 were assessed by immunohistochemistry.ANOVA test and t-test were performed for the statistical analyses.Results The tumor inhibition rates for the various treatments were：37.5%,Ma alone;75.0% DDP alone;83.3%,DDP＋Ma group DDP combined.The DDP＋Ma-induced inhibition was significantly greater than that achieved wit Ma or DDP alone （both P 〈 0.05）.The average weight of the DDP＋Ma group （21.5 g） was lower than that of the NS group （28.5 g） and the Ma group （26.67 g）,but higher than that of the DDP group （17.33 g）.In addition,the DDP＋Ma group also showed more robust general health,as indicated by activity,participation in life routines and appetite,than the DDP group.The rate of positive staining for survivin expression in tumor tissues was significantly lower in the DDP＋Ma group （19.58%±4.52%） than in the NS group （83.26%±15.56%）,the Ma group （62.50%±8.09%）,and the DDP group （38.67%±8.26%） （all P 〈 0.05）.In contrast,the rate of positive staining for Bax expression was significantly higher in the DDP＋Ma group （78.26%±6.09%） than in the NS group （21.15%±3.68%）,the Ma group （35.13%±10.57%）,a