中文摘要：

目的：检测人卵巢癌多细胞球体中p27和P—gp的表达。观察P27反义寡核苷酸（p27-ASON）抑制p27表达前后人卵巢癌多细胞球体多药耐药P-糖蛋白（P—gp）的表达，探讨P—gp蛋白表达与p27蛋白表达的相关性及p27反义寡核苷酸对卵巢癌细胞多药耐药的逆转作用。方法：以单层细胞为对照，以三维培养方法获得的人卵巢癌A2780、CAOV3多细胞球体（MCS）为模型，通过脂质体将p27-ASON转染入两卵巢癌细胞株，采用流式细胞仪（FACS）、蛋白质免疫印迹法（Western blot）检测p27、P—gp蛋白水平的表达；激光共聚焦显微镜（Confocal）检测p27、P-gp蛋白的亚细胞分布。结果：FACS、Western blot、Confocal检测提示单层细胞低表达p27，低表达P—gp，而在MCS中p27和P—gp表达明显升高。p27-ASON转染后培养的MCS，p27表达明显下调，同时P—gp表达下调。结论：卵巢癌多细胞球体中P—gp和p27的表达呈相关性．多药耐药性可能和p27的表达上调有关，p27-ASON可下调多细胞球体中P-gp的表达，从而可一定程度的逆转卵巢癌多药耐药性。

英文摘要：

Objective： To examine the expression of the cyclin-dependent kinase inhibitor p27 and P-glycoprotein （P-gp） in muhicellular spheroids （MCS） of ovarian cancer and to determine a potential method for reversing P-gp-mediated muhidrug resistance. Methods： A2780 and CAOV3 multicellular spheroids of ovarian cancer were obtained from three-dimensional culture and were used as models in this experiment. Both anti-sense （ASON） and sense-oligodeoxynucleotide （SON） were transduced into A2780 and CAOV3 cell lines by liposome. The expression of p27 and P-gp was detected using Western blot and flow cytometry （FACS）, and the sub-cellular distribution of p27 and P-gp was detected by laser confocal microscopy. Results： Compared with monolayer cells, the expression of both p27 and P-gp in MCS cells was significantly elevated. Low expression of P-gp was detected in monolayer cells, but the expression of P-gp in cell aggregates was significantly elevated. The p27-ASONmediated down-regulation of p27 decreased the level of P-gp protein. Conclusions： High expression of P-gp in ovarian cancer multi-cellular spheroids may be consistent with an increased level of p27. The p27-ASON may down-regulate the expression of P-gp, an effect that may be useful for reversing P-gp-mediated multidrug resistance.