中文摘要：

目的探讨ATP对大鼠背根神经节（DRG）神经元的电压门控性钙通道（VGCC）的抑制效应及其信号转导途径。方法在急性分离的大鼠DRG神经元上应用全细胞膜片钳技术记录ATP诱导的电流以及VGCC电流;应用钙离子成像技术检测胞内Ca^2＋浓度（[Ca^2＋]i）的变化。结果 ATP能够可逆性地抑制DRG神经元的VGCC电流和VGCC介导的[Ca^2＋]i升高。用PKA抑制剂H-89预处理DRG神经元,ATP对VGCC电流及其介导的[Ca^2＋]i升高的抑制被反转;而用PKC抑制剂bisindolylmaleimide（Bis）预处理DRG神经元,ATP对VGCC介导的[Ca^2＋]i升高的抑制效应则无影响。结论在大鼠DRG神经元中,PKA参与了ATP对该神经元VGCC的抑制。

英文摘要：

Objective To investigate the inhibitory effect of ATP on voltage-gated calcium channel （VGCC） currents and its signal transduction pathway in rat dorsal root ganglion （DRG） neurons.Methods Acutely isolated DRG neurons were used. Voltage-gated calcium channel （VGCC） currents and ATP-induced currents were recorded by patch clamp technique.Calcium imaging technique was applied to monitor the change of intracellular Ca^2＋ concentration （[Ca^2＋]i）.Results ATP could inhibit VGCC currents reversibly and the VGCC-mediated [Ca^2＋]i rise in rat DRG neurons. After pre-treated DRG with PKA inhibitor,H-89,the ATP-induced inhibitions on VGCC-mediated currents and [Ca^2＋]i rise were removed.Nevertheless,after pretreated DRG with PKC inhibitor,bisindolylmaleimide（Bis）,there was no effect on the ATP-induced inhibitions on VGCC-mediated [Ca^2＋]i rise.Conclusion PKA is involved in the inhibition of VGCC caused by ATP in rat DRG neurons.