中文摘要：

目的：应用蛋白质组学技术,开展中医肝肾阴虚证证候学研究。方法：对类风湿关节炎（RA）肝肾阴虚证、RA寒湿痹阻证、正常对照共3组各12例血清,分别进行双向凝胶电泳,通过软件分别比较肝肾阴虚证组和其他两组图谱,找出共同的肝肾阴虚证差异蛋白质点,应用质谱进行鉴定。结果：对10个肝肾阴虚证共同的差异蛋白点,进行鉴定和SwissProt数据库检索,得到6个肝肾阴虚证差异蛋白,涉及蛋白酶抑制剂、代谢相关酶蛋白、分泌多肽、运输蛋白等。结论：这些蛋白具有潜在的作为RA肝肾阴虚证诊断、预后标志物或治疗靶点的意义,也为进一步研究肝肾阴虚证实质提供了依据。

英文摘要：

Objective： Proteomic technique was applied to analyze rheumatoid arthritis （RA） patients with yin deficiency of liver and kidney syndrome. Methods： Serum proteins of RA patients with yin deficiency of liver and kidney syndrome group （12cases） were compared with that of normal control group （12cases） and RA patients with cold-dampness arthralgia spasm syndrome group （12cases） respectively by two-dimensional gel electrophoresis; common differential protein spots of yin deficiency of liver and kidney syndrome were analyzed by mass spectrometry. Results： 10 common differential protein spots were selected and 6 proteins were identified by mass spectrometry, which related to protease inhibitor, enzyme, secreted polypeptide, transport/cargo protein, and so on. Conslusions： These proteins are of potential significance in the diagnosis, prognostic markers, or treatment targets of RA patients of yin deficiency of liver and kidney syndrome, and also provide a basis for further studies of the essence of syndromes in traditional Chinese medicine.