中文摘要：

目的：基于蛋白质组学技术，开展强直性脊柱炎中医寒湿痹阻证的研究。方法：选取强直性脊柱炎中医寒湿痹阻证、湿热痹阻证、正常对照共3组血清各30例，对各组进行双向电泳，比较寒湿痹阻证组和其它两组电泳图谱，寻找共同差异蛋白点，质谱鉴定与数据库查询得到差异蛋白。结果：获得聚腺苷二磷酸核糖聚合酶1、弗林蛋白酶、血管内皮钙黏蛋白、激肽原1、血管紧张素原等5个强直性脊柱炎寒湿痹阻证差异蛋白，涉及酶类、粘附分子、凝血因子、肽类激素等。结论：这些蛋白可能涉及强直性脊柱炎寒湿痹阻证的发病和病理过程，为进一步研究其预后分子标志物、诊断以及治疗提供了依据。

英文摘要：

Objective： Proteomic technique was applied to analyze ankylosing spondylitis （AS） patients with cold-dampness arthralgia spasm syndrome. Methods： Serum proteins of AS patients with cold-dampness arthralgia spasm syndrome group （n= 30 ） was compared with that of normal control group （n= 30） and AS patients with damp-heat arthralgia spasm syndrome group （n= 30） respectively by two-dimensional gel electrophoresisl the common differential protein spots of cold dampness arthralgia spasm syndrome were analyzed by mass spectrometry. Results： Five common chlIerentlal protein spots were selected and identified by mass spectrometry. They are poly （ADP-ribose） polymerase 1, furin, VE-cadherin, kininogen 1, angiotensinogen and related to enzyme, adhesion molecule, coagulation factor, peptide hormone, and so on. Conclusion： These proteins are implicated in the pathogenesis and pathological processes of AS with cold-dampness ar- thralgia spasm syndrome, and provide a basis for further studies of the diagnosis, prognostic markers, or treatment of AS patients with cold dampness arthralgia spasm syndrome.