中文摘要：

为探究不同昆虫可溶性海藻糖酶的潜在功能,在获得褐飞虱2个可溶性海藻糖酶NlTRE1-1和NlTRE1-2的cDNA全长序列的基础上,采用生物信息学相关软件分析其序列特征以及二级、三级结构等分子特性及生理功能,同时构建不同昆虫海藻糖酶的系统进化树,进一步采用RNAi技术分析单个海藻糖酶基因抑制效果.结果显示,褐飞虱可溶性海藻糖酶NlTRE1-1和NlTRE1-2蛋白相似性高,其蛋白质二级和三级结构均类似,二级结构中含有相似比例的α螺旋、β-折叠和无规卷曲;系统进化树分析表明褐飞虱海藻糖酶与大豆蚜、豌豆蚜和灰飞虱等昆虫的TRE1具有较近的亲缘关系;且两个褐飞虱特异性TRE1-1和TRE1-2的dsRNA注射后48h,本基因的表达极显著下降.研究结果为以抑制海藻糖酶基因表达来进行的害虫防治技术奠定基础.

英文摘要：

In order to explore the potential functions of different insects＇ soluble trehalase, basing on the full-length sequence of two soluble trehalase N1TREI-1 and N1TRE1-2 cDNAs, the sequence characteristics as well as the secondary and tertiary structures of two TRE1 in Nilaparvata lugens were analyzed using the bioinformatics software. Then, the phylogenetic tree of trehalase from different insects was constructed. And the effect of single trehalase gene by RNAi technique was studied. These results showed that the soluble trehalase NITRE1-1 and N1TRE1-2 proteins had high similarity, their protein secondary and tertiary structures were similar. The secondary structure contained similar ratio a-helix, 13-sheet and random coil. The phylogenetic tree analysis showed that two trehalase of N. lugens had a close genetic relationship with the TRE1 of Aphis glycines, Acyrthosiphon pisum and Laodelphax striatella. The expression of this gene was significantly decreased when the two specific TRElq and TRE1-2 dsRNAs were injected 48 h. This study results provided a basis for the prevention and treatment of pests that inhibited trehalase gene expression.