采用水稻敏感品系T N1和抗性品系中浙优、IR56饲养褐飞虱,研究褐飞虱产卵量等繁殖情况,测定解毒酶系中酯酶、细胞色素P450和谷胱甘肽转移酶(GST )及卵黄原蛋白(Vg )等基因在mRNA上的表达变化。结果显示褐飞虱在抗性水稻上产卵量减少,Vg2的表达量极显著下降,表明水稻抗性对褐飞虱的产卵量有抑制作用且Vg2调控产卵。酯酶和P450中的部分基因均在取食抗性水稻的褐飞虱体内表达量较高,存在极显著差异。
This experiment adopts the TN1 sensitive rice varieties and other different levels of resistance rice cultivars , such as Zhongzheyou and IR56 ,to breed N .lugens ,and then use a part of those N .lugens to study N .lugens spawning and other breeding situations ,and determines the expression changes of esterase ,cytochrome P450 ,Glutathione transferase (GST) ,vitellogenin(Vg) on mRNA .The results show that the fecundity of N .lugens reduces on resistance rice ,and the expression of Vg2 decreases significantly ,which indicates resistance rice has inhibitory effect on N . lugens fecundity and Vg2 regulate the spawning .Part genes of esterase and P450 have high expression in the resistance rice N . lugens body , which have significant differences .