中文摘要：

目的制作大鼠青光眼滤过手术模型，对以往建立的动物模型进行补充，以更好地开展青光眼滤过手术的相关研究。方法SD大鼠10只，制作以角膜缘为基底的结膜瓣，通过全巩膜隧道穿入前房25号硅胶管，末端埋于结膜下，连续缝合结膜瓣。同样方法做另外3只大鼠，分别于术后2、5、11d取眼球做组织学检查，观察术后滤过泡形成情况。结果通过大鼠前房引流管植入术，可以在结膜下形成良好的滤过泡，经过7～11d逐渐瘢痕化。结论大鼠引流管植入术是一个可靠的青光眼滤过手术动物模型，可用于滤过手术后伤口愈合、瘢痕化过程及滤过手术其他方面的相关研究。

英文摘要：

Objective The rat is an ideal animal as a model of general,corneal and retinal wound healing,but rabbit has been used as a standard animal model for wound healing and scarring process following glaucoma filtering surgery. Difference from rat, mouse and non-human primate models, the genetic sequences of rabbits for this type of work has not been well defined. The purpose of this investigation was to establish a modified operative model of glaucoma filtering surgery. Methods Glaucoma filtering surgery was performed on the right eyes of 10 Sprague-Dawley rats by introducing a 29-gauge silicone cannula through a penetrating scleral tunnel under a limbal-based conjunctival flap. Identical glaucoma filtering surgery was performed on other 3 rats for the histologic evaluation at day 2,5,11 following surgery. Results Following the cannula of glaucoma filtering surgery, well-elevated filtering blebs formed and gradually failed over the course of 8 to 13 days. The sub-conjunctional bleb was elevated and avascular in 24 hours after surgery and gradually flatten and vascularized 5 days later. The inflammatory cells was gradually increased around the bleb in the 1 -5 days of postoperation and decreased 11 days later and the connective tissue was gradually density with lapse of postoperation time. The intraocular pressure was lowest in the day I after operation and returned to the baseline level in 5 and 6 days postoperatively. No any complication occurred in model rat after operation. Conclusion Rat model of cannulating filtering surgery offers a long lasting and predictable method for the studies of wound healing following glaucoma filtering surgery and induced changes at the gene level.