中文摘要：

背景 视觉发育可塑性与弱视治疗时间和方法的选择密切相关,synapsin（突触素）作为一种突触前特异性蛋白家族,在视觉发育可塑性中的作用尚未明确. 目的 动态观察正常小鼠视皮层内synapsin蛋白表达水平（T-synapsin）及其磷酸化水平（p-synapsin）随生长和发育发生的量的变化.方法 选用清洁级健康新生C57BL/6小鼠42只,分别于出生后第7、14、21、28、35、42、60天取左右两侧视皮层,每个时间点选6只小鼠.采用Western bolt法检测不同鼠龄小鼠视皮层内不同亚型synapsin的表达量及其位点1的磷酸化水平. 结果 正常C57BL/6小鼠视皮层中synapsin的表达随生长和发育发生动态变化,出生后第7、14、21、28、35、42天,小鼠视皮层中T-synapsin Ⅰ a/b的表达量分别为成年小鼠（出生后第60天,P60）表达量的（21.32＆#177;3.27）％、（56.27＆#177;10.18）％、（77.05＆#177;10.05）％、（83.75＆#177; 10.52）％、（94.69＆#177;11.46）％和（98.75＆#177;5.86）％,不同鼠龄小鼠视皮层中T-synapsin Ⅰ a/b表达量的总体比较差异有统计学意义（F=69.538,P＜0.001）,其中P7、P14、P21、P28组表达量明显低于成年组,差异均有统计学意义（P＜0.05）,P35、P42组与成年组相比差异均无统计学意义（P=0.280、0 798）;不同亚型T-synapsin在视皮层中的表达趋势随小鼠的生长和发育略有不同,其中T-synapsinⅡa、Ⅲa增长相对缓慢,P60时仍呈增长趋势,不同鼠龄间T-synapsinⅡa、Ⅱb、Ⅲa表达的总体比较差异均有统计学意义（F=42.492、55.595、39.172,P＜0.001）;p-Synapsin Ⅰ a/b的表达在出生后随小鼠的发育而逐渐增高,P21左右达高峰,为成年小鼠磷酸化水平的（2.86＆#177;0.17）倍,此后迅速下降,成年后维持低磷酸化水平,不同鼠龄间小鼠视皮层中p-synapsin Ⅰ a/b表达量的总体比较差异有统计学意义（F=22.620,P＜0.001）.结论 小鼠视皮层中synapsin的表达量随生长和发

英文摘要：

Background The treatment timing and method of amblyopia rely on the plasticity of visual system.Synapsin is a family of presynaptic terminal specific protein.Its role in visual developmental plasticity is below understood.Objective To investigate the dynamic expressions of synapsin （T-synapsin）,and phosphorylation of synapsin （p-synapsin Ⅰ a/b） in visual cortex of normal mice and further explore the role of synapsin in plasticity of visual system.Methods Forty-two clean neonatal C57BL/6 mice were collected.The mice were sacrificed at postnatal 7,14,21,28,35,42,60 days respectively to obtain the tissue samples of visual cortex.Expression levels of T-synapsin and p-synapsin in the visual cortex following the ageing were quantitatively detected using Western blot assay.Results The expression of synapsin in normal mice showed a dynamic increase with the ageing.The T-synapsin Ⅰ a/b/β-actin value in visual cortex was （21.32 ＆#177; 3.27） %,（56.27 ＆#177; 10.18） %,（77.05 ＆#177; 10.05） %,（83.75＆#177;10.52） %,（94.69＆#177;11.46）%,（98.75＆#177;5.86） % of adults mice （postnatal 60 days,P60） in the mice of postnatal 7,14,21,28,35,42 days,respectively,showing a significant difference among them （F =69.538,P 〈 0.001）.Compared with the adult mice,the T-synapsin Ⅰ a/b/β-actin value in the mice of P7,P14,P21,P28 was significantly lower （all at P〈0.05）,but no significant difference was found between P35 and P60,P42 and P60 （P =0.280,0.798）.The development trend of different synapsin subtypes,such as T-synapsin Ⅰ a/b,T-synapsin Ⅱ a,T-synapsin Ⅱ b and T-synapsin Ⅲ a,was not quite the same during the ageing.The expression of T-synapsin Ⅱ a and Ⅲ a increasing more slowly with development,and kept increasing until P60.Significant differences were found among various age of mice in T-synapsin Ⅱ a,Ⅱ b,Ⅲa respectively（F =42.492 55.595,39.172,all at P〈0.001）.The p-synapsin Ⅰ a/b level in the visual cortex elevated with the ageing of the mice,and t