中文摘要：

目的：探索中药柴贝止痫汤对大鼠离体脑微血管内皮细胞膜上P糖蛋白（P-gp）及多药耐药基因1（Mdr1）mRNA表达的影响。方法：培养原代大鼠脑微血管内皮细胞,传至3代时用2mmol/L的L-谷氨酸作用脑微血管内皮细胞3h,诱导其细胞膜上P-gp/Mdr1表达增加,模拟难治性癫痫血脑屏障P-gp的升高。MTT法检测柴贝止痫汤的安全药物浓度范围,并设4个浓度梯度（10、100、500μg/mL、1mg/mL）干预模型细胞24h;Western Blot法检测P-gp的表达,Real-time PCR检测Mdr1 mRNA的扩增。结果柴贝止痫汤100、500μg/mL组P-gp的表达较模型组显著降低（P〈0.05）;柴贝止痫汤100μg/mL、500μg/mL组Mdr1a mRNA的表达较模型组显著降低（P〈0.05）,500μg/mL组Mdr1b mRNA的表达较模型组显著降低（P〈0.05）。结论：柴贝止痫汤可以下调谷氨酸诱导的大鼠脑微血管内皮细胞P-gp/Mdr1表达。

英文摘要：

Objective： To observe the effects of Chaibei Zhixian Decoction on regulating the expressions of P-glycoprotein（P-gp） and Multi-drug resistant gene 1（Mdr1） in rat brain microvascular endothelial cells.Methods： Primary brain microvascular endothelial cells were cultured in vitro.Then,the 3rd generation cells were exposed to L-glutamate（2mM） for 3 hours to induce the overexpression of P-gp/Mdr1 on the cell membranes,which copied the overexpression of P-gp /Mdr1 in blood brain barrier（BBB） when refractory epilepsy attacked.MTT assay was used to detect the safe range of Chaibei Zhixian Decoction concentration.The model cells were intervened with different concentrations of Chaibei Zhixian Decoction at 10,100,500μg/m L and 1mg/m L,lasting for 24 hours.Western Blot was used to detect the expression of P-gp and Real-time PCR was used to determinate the amplification Mdr1 mRNA.Results： The P-gp expressions in 100μg/m L concentration of Chaibei Zhixian Decoction group and in 500μg/m L concentration of Chaibei Zhixian Decoction group were both significant lower than that in the model group（P〈0.05）.The mRNA expressions of Mdr1 a in 100μg/m L and 500μg/m L concentration of Chaibei Zhixian Decoction groups were both significant lower than that in model group（P〈0.05）,as well as the mRNA expressions of Mdr1 b in 500μg/m L concentration of Chaibei Zhixian Decoction group.Conclusion： Chaibei Zhixian Decoction could down-regulate the expressions of P-gp and Mdr1 mRNA in rat brain microvascular endothelial cells exposed by L-glutamate.