为了比较刺槐不同无性系在延庆地区的生长表现,选取匈牙利、韩国、中国河南及延庆本地刺槐优良无性系11个,于2008年4月种植在北京延庆县。在生长季测定各无性系的净光合速率和叶片的叶绿素、可溶性糖等生化指标,并且连续3年测定各无性系的株高、地径等表型性状。结果表明:匈牙利刺槐H2、韩国刺槐K2、韩国刺槐K4、河南刺槐8048和延庆本地刺槐2N的株高、地径表现较好,这些无性系株高、地径3年的数据经多重比较分析后均被归为数值最高的一类;净光合速率较高的无性系为匈牙利刺槐H2(11.78±0.24)μmol/(m2·s)、河南8044(12.57±0.21)μmol/(m2·s)和延庆本地刺槐2N(12.92±0.46)Izmol/(m2·s);可溶性糖、还原性糖和自由氨基酸含量最高的无性系为河南84023(68.97±8.61)mg/g、韩国K2(111.69±6.85)mg/g和河南8048(426.34±5.43)μg/g;叶绿素a、叶绿素b含量最高的无性系都是匈牙利刺槐H2,其均值分别为(3512.33±38.27)μg/g和(3824.19±118.21)μg/g;类胡萝b素含量最高的无性系为匈牙利刺槐YDL(532.67±13.99)μg/g;除叶绿素a、b和第一年所测株高显著相关(P〈0.05)外,其他生化指标和净光合速率与3年所测表型指标没有显著的相关性。综合分析表明,匈牙利刺槐H2、韩国刺槐K2、韩国刺槐K4、河南刺槐8048和延庆本地刺槐2N无性系在延庆地区生长较好,目前无性系选优还主要依靠株高、地径等表型指标的测定。
Eleven elite clones of Robinia pseudoacacia, selected from Hungary, South Korea, Henan Province and the local county Yanqing of Beijing , were planted in Yanqing County in April, 2008 to investigate their growth performance in Yanqing Region of Beijing. Physiological and biochemical traits, such as net photosynthetic rate, chlorophyll and soluble sugar contents were measured. In addition, multiple morphological characters, such as height and basal diameter had been annually recorded for 3 years. Tukey's multiple comparison analysis indicated that clones of Hungary H2, South Korea K2 and K4, Henan 8048, and a local Yanqing 2N were superior in height and basal diameter, which distinguished them from other clones. Yanqing 2N (12.92±0.46) μmol/(m2.s), Henan 8044 (12.57 ±0.21)μmol/( m2·s), and Hungary H2 (11.78 ± 0.24) μol/( m2- s) showed higher net photosynthetic rate than others. Henan 84023, South Korea K2, and Henan 8048, in that order, had the highest content of soluble sugars (68.97 ± 8.61 ) rag/g, reducing sugars ( 111.69 ± 6.85) mg/g, and free amino acid (426. 34_± 5.43) μg/g. Hungary H2 clone had the highest contents of both chlorophyll a and chlorophyll b,and the mean values were (3 512.33 ± 38.27) and (3 824. 19 ± 118.21)μg/g ,respectively. The highest carotenoid content was found in Hungary YDL (532.67 ± 13.99)μg/g. Except for a significant correlation (P 〈 0.05) between chlorophyll content and the first year plant height, analysis showed no significant correlations between other physiological or biochemical traits and morphological characters. The results suggest that Hungary H2, South Korea K2 and K4, Henan 8048 and the local Yanqing 2N are the most recommended R. pseudoacacia clones for plantations in Yanqing County of Beijing. The current clone selection still highly depend on the measurement on plant height, basal diameter and other morphological characters.