中文摘要：

以A188玉米自交系的愈伤组织为材料，研究了影响农杆菌介导玉米转化和再生的各种因素，建立了该自交系的遗传转化体系。结果表明，1．0—1．5mm的幼胚最易诱导出胚性愈伤组织；在侵染液和共培养基中加入乙酰丁香酮能显著提高转化效率；合适的菌液浓度（OD600=0．4）、共培养时间（3d）、共培养温度（24℃）有利于提高转化效率。

英文摘要：

The factors affecting the Agrobacterium tumefciens - mediated transformation and regeneration of maize （Zea mays L. ） embryogenic callus were optimized, and the genetic transformation system was established for the maize inbred line A188. The results indicated that 1.0 - 1.5 mm immature embryos were easier to induce embryogenic callus; the addition of acetosyringone in infection solution and common medium could increase the transformation efficiency significantly; appropriate bacterial concentration （OD600 = 0.4）, incubation time （3 days） and co- culture temperature （24℃） were benefit to increasing formation efficiency.