中文摘要：

背景：膀胱运动的效应细胞是逼尿肌细胞,而膀胱Cajal间质细胞如能在膀胱运动中作为起搏细胞而存在,必须能对逼尿肌细胞进行调控。因此,二者在结构和功能上的联系可以从一个方面证明Cajal间质细胞是否具有起搏细胞地位。目的：观察膀胱逼尿肌细胞与Cajal间质细胞结构和功能上的联系,分析膀胱Cajal细胞在逼尿肌细胞兴奋调控中的作用。设计、时间及地点：观察对比实验,于2007-04/10在解放军第三军医大学基础部中心实验室完成。材料：选用SD大鼠用于取膀胱逼尿肌新鲜组织块。方法：应用透射电镜观察Cajal间质细胞和逼尿肌细胞之间的超微结构联系;利用荧光漂白技术模拟信号从Cajal间质细胞传递到逼尿肌细胞的过程,观察Cajal间质细胞与逼尿肌细胞之间的信号交流情况。主要观察指标：Cajal间质细胞和逼尿肌细胞之间的超微结构联系及荧光强度变化。结果：①Cajal间质细胞和逼尿肌细胞之间有缝隙连接。②对单个Cajal间质细胞相邻的逼尿肌细胞进行荧光漂白后,发现逼尿肌细胞内的荧光强度明显恢复,同时Cajal间质细胞的荧光强度逐渐减弱。说明Cajal间质细胞与逼尿肌细胞之间有信号通讯通路。结论：逼尿肌细胞与Cajal间质细胞之间存在结构上的联系,Cajal间质细胞可以将兴奋信号传递给周围的逼尿肌细胞。

英文摘要：

BACKGROUND： Detrusor smooth muscle cells are effector cells in the bladder movement. If interstitial cells of Cajal （ICC） in the bladder can serve as pacemaker cells, ICC should be regulated. Thus, their correlation in relation to structure and function can verify whether ICC can serve as pacemaker cells. OBJECTIVE： To investigate the structural and functional relationship between detrusor smooth muscle cells and ICC in the bladder and to analyze the effect of ICC in regulating the excitability of detrusor smooth muscle cells. DESIGN, TIME AND SETTING： The control observation experiment was performed at the Central Laboratory, Basic College, Third Military Medical University of Chinese PLA from April to October 2007. MATERIALS： Sprague-Dawley （SD） rats were used to collect fresh tissue blocks of detrusor smooth muscle cells in the bladder. METHODS： Ultrastructural connection between detrusor smooth muscle cells and ICC in the bladder was detected with a transmission electron microscope. Signal transmission from ICC to detrusor smooth muscle cells in the bladder was simulated by fluorescent bleach technique. Signal exchange was observed between ICC and detrusor myocytes. MAIN OUTCOME MEASURES： Ultrastructure connection and fluorescence intensity between ICC and detrusor smooth muscle cells. RESULTS： Gap junction was confirmed between ICC and detrusor smooth muscle cells. After fluorescent bleach on target detrusor smooth muscle cells, fluorescence intensity in detrusor smooth muscle cells was significantly recovered. In the meantime, fluorescence intensity in the ICC neighboring to the target detrusor smooth muscle cells was decreased. This indicates a signal transmission from ICC to detrusor smooth muscle cells. CONCLUSION： There is a structural connection between ICC and detrusor smooth muscle cells. Excitability signals may be transferred from ICC to detrusor smooth muscle cells.