中文摘要：

吉本斯们在进化期间经历了广泛的 karyotype 重新整理并且为学习进化 chromosomal 重新整理的内在的分子的机制代表一个理想的模型。克隆和进化 chromosomal 断点的顺序描述将提供重要卓见进驾驶了如此的激进的 karyotype 的分子的力量，这被期望在长臂猿改组。我们构造了并且描绘非包含 192,000 的白脸颊的长臂猿(Nomascus leucogenys ) 的一个高质量的 fosmid 图书馆 -- 有 38 kb 和 2.5 褶层染色体范围的一种平均 insert 尺寸的冗余的克隆。到 100 随机选择的 fosmid 克隆定序的结束，我们为图书馆产生了 196 个顺序标签。这些定序结束的 fosmid 克隆然后被荧光在 situ 杂交印射到白脸颊的长臂猿的染色体上，并且没有假妄想的克隆被检测。对人的染色体的强风搜索显示出在点击克隆的数字和染色体的数字之间的好关联， fosmid 图书馆的不偏的 chromosomal 分发的一个指示。印射的克隆的 chromosomal 分发与对人、白脸颊的长臂猿染色体的 BLAST 搜索结果也一致。fosmid 图书馆和印射的克隆愿望在更小的无尾猿为进一步学习长臂猿的 chromosomal 重新整理和内在的分子的机制以及为比较 genomic 学习用作一个珍贵资源。

英文摘要：

Gibbons have experienced extensive karyotype rearrangements during evolution and represent an ideal model for studying the underlying molecular mechanism of evolutionary chromosomal rearrangements. It is anticipated that the cloning and sequence characterization of evolutionary chromosomal breakpoints will provide vital insights into the molecular force that has driven such a radical karyotype reshuffle in gibbons. We constructed and characterized a high-quality fosmid li- brary of the white-cheeked gibbon （Nomascus leucogenys） containing 192,000 non- redundant clones with an average insert size of 38 kb and 2.5-fold genome coverage. By end sequencing of 100 randomly selected fosmid clones, we generated 196 se- quence tags for the library. These end-sequenced fosmid clones were then mapped onto the chromosomes of the white-cheeked gibbon by fluorescence in situ hy- bridization~ and no spurious chimeric clone was detected. BLAST search against the human genome showed a good correlation between the number of hit clones and the number of chromosomes, an indication of unbiased chromosomal distribu- tion of the fosmid library. The chromosomal distribution of the mapped clones is also consistent with the BLAST search result against human and white-cheeked gibbon genomes. The fosmid library and the mapped clones will serve as a valu- able resource for further studying gibbons＇ chromosomal rearrangements and the underlying molecular mechanism as well as for comparative genomic study in the lesser apes.