中文摘要：

目的：检测系统性红斑狼疮患者外周血中CD19＋细胞数目的变化及p16mRNA的表达。方法：采用免疫磁珠法分离SLE外周血CD19＋细胞并计数；分选出来的细胞提取RNA，应用实时定量lit—PCR方法检测CD19＋细胞中p16mRNA表达水平。结果：SLE患者外周血中CD19＋细胞（66．23±4．455）较正常对照组（32．15±5．108）增加。SLE患者p16mRNA在CD19＋细胞中的表达（47．3731±22．2939）比健康对照组（0．7276±0．1183）明显增加。结论：CD19＋细胞的数量及p16mRNA的异常在SLE的发病中起一定作用。

英文摘要：

Objective： To assess the quantity of peripheral blood of CD19＋ ceils and detect the expression of p16 mRNA on peripheral blood of CD19＋ ceils in the patients with systemic lupus erythematosus （SLE）. Methods： CD19＋ cells were separated from the peripheral blood using immunomagnetic beads and counted in 30 SLE patients and 20 hea/thy controls. Reverse transcription - pelymerase chain reaction （RT - PCR） technique was applied to quantitatively analyze the expression of p16 mRNA in CD19＋cells. Results： Compared with controls, the quantity of CD19＋ cells and the level of p16 mRNA expression in CD19＋ cells were significantly increased in SLE patients. Conclusion： The abnormality in the quantity of CD19＋ cells and the expression of p16 may play a role in the patho- genesis of SLE.