中文摘要：

目的：探讨p16基因mRNA在系统性红斑狼疮（SLE）患者CD4^＋、CD8^＋T细胞中的表达。方法：收集40例确诊的SLE患者（患者组）和30名正常人（对照组）外周血，应用免疫磁珠法分选CD4^＋、CD8^＋细胞并计数，提取RNA，应用实时定量RT—PCR方法检测外周血CD4^＋、CD8^＋T细胞中P16基因mRNA表达水平。结果：①p16基因mRNA在CD4^＋T细胞中的表达：分别与对照组（2．245±0．586）和SLE非活动期组（1．361±0．154）相比较，SLE活动期组（93．264±42．898）明显增加，差异均具有统计学意义；而非活动期组与对照组之间差异无统计学意义。②p16基因mRNA在CD8CF细胞中的表达：分别与对照组（4．323±0．735）和非活动期组（3．722±1．701）比较，SLE活动期组（92．926±38．102）明显增加，差异均具有统计学意义；而非活动期组和对照组之间差异无统计学意义。结论：SLE患者p16基因mRNA水平在CD4^＋,CD8^＋T细胞中增高，提示SLE患者存在p16基因表达异常。

英文摘要：

Objective： In this study we assessed the expression of p16 mRNA of peripheral blood CD4^＋, CD8^＋T cells in active or inactive SLE. Methods： Forty definitive SLE patients and 30 healthy controls were collected. CD4^＋, CD8^＋cells were separated from the peripheral blood using immunomagnetic beads and counted. Reverse transcriptiou-polymerase chain reaction （RT-PCR） technique was applied to quantitatively analyze the expression of p16 mRNA in CD4^＋, CD8^＋ cells. Results：Level of p16 mRNA expression in CD4^＋ cell was significantly increased（93.264±42.898）in active patients with SLE as compared with inactive（ 1.361±0.154）and controls （2.245±0.586）. Level of p16 mRNA expression in CD8^＋ cell was significantly increased（92.926±38.102） in active patients with SLE as compared with inactive （3.722±1.701）and controls （4.323±0.735）. Conclusion： These results suggest that the expression of p16 gene is abnormal in SLE patients.