中文摘要：

目的探讨兔软骨细胞损伤后基质金属蛋白酶（matrix metalloproteinase，MMP）-13与聚集蛋白聚糖（aggrecan，AGG）、Ⅱ型胶原（type—Ⅱ collagen，Col-Ⅱ）mRNA的相互作用。方法2014年9至12月，30只5月龄健康雄性新西兰大白兔依次标号分为对照组（自体软骨移植）、假手术组（阻断周围营养的软骨移植）、手术组（阻断软骨关节营养液），每组10只；造模后4周，处死兔取标本行大体、组织学观察；采用实时荧光定量PCR检测MMP-13、AGG和Col-ⅡmRNA的表达情况；采用TUNEL凋亡染色检测细胞凋亡率；对细胞凋亡与软骨组织学Mankin评分及MMP-13、AGG、Col-ⅡmRNA相对表达进行相关性分析。结果手术组[（7．20±1．22）分]与对照组[（1．20±0．29）分]Mankin评分比较，差异有统计学意义；假手术组[（1．10±0．31）分]与对照组比较差异无统计学意义。造模后4周，手术组细胞凋亡率（18．43％±3．86％）明显高于对照组（4．56％±0．93％），假手术组（4．61％±0．93％）与对照组比较差异无统计学意义；手术组MMP-13mRNA的表达高于对照组，而AGG和C01．ⅡmRNA的表达低于对照组，假手术组与对照组比较差异无统计学意义；Mankin评分与细胞凋亡率呈正相关（r=0．922，P〈0．001），回归方程：Y=-0．548＋0．404X（R^2=0．844，F=157．735，P〈0．001）；MMP．13mRNA表达量与细胞凋亡率呈正相关（r=0．942，P〈0．001），回归方程：Y=0．951＋0．116X（R^2=0．883，F=219．054，P〈0．001）；MMP-13mRNA的表达与AGG、Col-Ⅱ的mRNA表达呈负相关（r=-0．956、-0．945，均P〈0．001）。结论软骨细胞损伤所促发的细胞凋亡可以通过上调MMP-13的表达来加剧软骨细胞的退变，进而导致AGG、Col—ⅡmRNA表达下降，导致细胞外基质合成紊乱。

英文摘要：

Objective To explore the relationship among the expression level of MMP- 13, AGG and Col-Ⅱ in the chondrocytes caused by nutritional deficiencies in rabbits. Methods 30 New Zealand white rabbits were randomly divided into autologous chondrocyte transplantation group （control group, n=10）, nutrition block group （surgery group, n=10）, and peripheral nutrition block group （sham surgery group, n=10）. 4 weeks after treatment, the rabbits were sacrificed for undergoing the observations on general and histological level; real-time PCR assay was employed for testing the expression level of MMP- 13, AGG and Col-Ⅱ; cellular apoptosis percentage was observed through TUNEL stain. The relationship among the apoptosis level, cartilage cells histological Mankin score as well as the expression level of MMP- 13, AGG and Col-Ⅱ were analyzed. Results Based on the Mankin score, there was a statistic difference between surgery group and control group. On the other side, there were no statistic differences between sham surgery group and control group. 4 weeks after treatment, surgery group presented a higher apoptotic percentage compared with control group; this value between sham surgery group and control showed no significant differences. There was an increased mRNA expression level of MMP-13 and a decreased mRNA expression level of AGG and Col-Ⅱ in surgery group com- pared with control group; no statistic differences of all these values was found between sham surgery group and control group. Histological Mankin score and apoptotic percentage presented positive correlation （r=0.922, P 〈 0.001）, the regression equation： Y=-0.548＋ 0.404X, R^2=0.844 （F=157.735, P 〈 0.001）; the mRNA expression level of MMP-13 and apoptotic percentage presented positive correlation （r=0.942, P 〈 0.001）, the regression equation： Y=0.951 ＋0.116X, R^2=0.883 （F=219.054, P 〈 0.001）. There was a nega- tive correlation between the mRNA expression level of MMP- 13 and the mRNA expression level of AGG as well as